In my MTT assay I make control with medium with 0.5% of ethanol, the maximum quantity to put on the cells.
Then, in the different concentrations of extracts, I have used lower concentrations of ethanol. However, I Know that the absorbance values have an effect on plant extracts and ethanol. How I can obtain the absorbance values only of the extracts, without the effect of the ethanol?
Dear Ana Paço, You can use wells on well plates that contain only medium and ethanol/methanol (Mo), medium + ethanol + extract dose 1 (M1), and so on (all without cell line) as a control, so each dose of extract has its own control.
Check http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300011
Also Check
https://www.researchgate.net/deref/http%3A%2F%2Fwww.bioline.org.br%2Fpdf%3Fpr15260
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