How high-quality Fetal Bovine Serum from BioWest for cell cultures ?

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Are formal ethical approvements must haves for social-psychology research?

My sociological research considers emotions. Searching for the journal to submit, I have found that some journals ask to provide the information considering the formal approvment of the research...

14 May 2023 6,191 12 View

Can ham beetles be used to strip off meat of the bones of the specimens to be put in a museum?

We have lost all of our dermastids due to fly larvae. After a month we had ham beetles appearing and I was wondering if they would do as good of a job to get rid of meat from bones, so that we can...

26 March 2020 7,590 3 View

Protocol for microwave irradiation in immunofluorescence staining?

I am having problems with non-specific antibody binding in IF. I came across this method of antigen retrieval using microwave irradiation. The problem is that some use it after permeabilization...

21 March 2019 6,452 1 View

If I machine wash possibly contaminated by liquid mercury bedsheets are they decontaminated?

After mercury spill from a broken mercury thermometer on the carpet of my bedroom.

23 June 2018 6,091 3 View

Are there any relatively inexpensive methods of measuring sedimentation in sea water (up to 10 metres deep)?

We are trying to see the effect of sedimentation on wildlife in Tanzania and we can't seem to find any effective but yet inexpensive way of measuring sedimentation. any advice would be much...

21 May 2015 8,482 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View