Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why there is no increase in virus titer after 24 hour of seeding for harvest?

I am working on virus production through bioreactor,my concern is regarding ,no increase in virus titer after 24 hour of seeding for harvest Usually we seed the fermenter and incubate for 72...

05 January 2021 3,860 2 View

Can anyone suggest how to remove H&E stain from the stained slide?

Dear friends i have only H&E stained slide and i have to do other special staining from this slide only. kindly suggest me the procedure for complete destaining of this slide.

13 December 2020 634 4 View

How many cells would be good to perform Chip seq for FoxP3 binding domain? Which fixative would be good for the same?

We are trying to find FoxP3 interactive domain in WT vs KO mouse regulatory T cells (Treg) through Chip Seq analysis. I am unable to get anything after pulldown although sonitation was good....

24 September 2020 244 4 View

How to identify fake research and predatory journal?

Fake researches and predatory journals

14 September 2020 4,530 7 View

Which professional society bodies in India related to Civil Engineering provide worthy periodicals for researchers/Engineers?

List out the best professional bodies which provide worthly and subject-related periodicals. Also, list out as per specializations like Structural Engineers, Geo-Technical Engineers, Environmental...

09 September 2020 1,151 11 View

Does anyone aware of a easy way to extract data on the number of publications per year on a particular research area?

Is there any easiest way? I need data of my research area for my upcoming review article.

12 August 2020 3,826 4 View

How to report ordinal regression ?

The method of analysis for my research is ordinal regression contains 6 ranked variables. But the goodness of shows unexpected and i am confused. Is this really possible, my data fit well or...

29 July 2020 2,291 3 View

Does web of science cover all PubMed indexed journals or both have different list of journal (May be some of same)?

24 July 2020 4,802 3 View

Why we did not use normal glass (silica glass)in the surface plasmon resonance(SPR) based sensor ??

I have designed a SPR chip by using normal glass slide(Blue star No. 4slide)/Cu(5nm)/Ag(50nm). But it shows very less depth in the reflectance curve at resonance angle. What should be the reason ??

13 February 2020 3,770 4 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

How to indicate the degree of litter decomposed by microbes?

Any technique could detect the degree of litter decomposed by microbes? Or any indexes could do this job? I want to split the contribution of microbes during decomposition, so any suggestions...

23 February 2021 1,362 5 View

Universal bacterial primers targeting a mRNA?

Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...

22 February 2021 9,969 3 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

Can I get information about research laboratories that are currently having in culture, internationally/locally standardised microorganisms?

Microorganisms of interest include; E.coli; Salmonella typhimurium; Pseudomonas aeroginosa; Streptoccocus pneumoniae; Staphyloccocus aureus and Candida albicans. Locations closer to Port...

18 February 2021 6,500 3 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View