How to do the calculation for super oxide dimutase assay and how to calculate the R squared value in regression equation for catalase assay?

i have the enzymatic antioxidant assay by following the method of Misra and Fridovich, 1972 for super oxide dimutase and Luck, 1974 for catalase assay can any help me how to the calculation for...

11 February 2021 2,241 3 View

How to find the dihedral angle of particular residue over the period of simulation time?

Dear Friends, I would like to perform the dihedral angle calculation of particular residue over the period of simulation time. Could you please help me out in creating the ndx file using make_ndx...

19 March 2018 2,284 3 View

How to perform the PIEDA calculation for the receptor-ligand residues using FMO ?

Dear All, Im trying to compute the PIEDA for the receptor-ligand residues using the FMO calculation. Could you help me in providing the steps to create the input put file for running the...

10 November 2017 144 2 View

How to calculate the host-guest binding affinity using quantum mechanics

16 June 2017 1,904 3 View

Which is the alternate animal model for type II collagen induced arthritis in DBA/1 mice?

Is there any alternate animal model for type II collagen induced arthritis in DBA/1 mice? DBA/1 mice is not available easily and thus suggestion on any preferred animal model for the study is...

19 December 2016 5,003 2 View

Can anyone suggest the best chromatographic technique to be adopted to well separate high molecular weight tannins?

Used methods in the literatures include SEC and Prep.HPLC.

10 June 2014 1,018 3 View

How HOMA - IR can be evaluated in rodent models? I read in few articles that HOMA is not validated in animals. is it so?

The HOMA is calculated from the product of plasma glucose and insulin levels divided by a constant. In animal models the mean plasma glucose and insulin levels vary according to the species....

01 January 1970 6,215 14 View

Extraction of phytochemicals from juice?

I am trying to extract phytochemicals from cane juice. I have tried lyophilization and SALLE techniques but the final product is too sticky and sugars are hindering any further extraction. I have...

03 February 2021 8,784 3 View

How to screen phytochemicals directly from the crude plant latex?

Number of methods are available to screen phytochemicals through extract prepared in different solvents including aqueous and organic. Is there any method/ published work related to phytochemical...

01 February 2021 7,348 4 View

I have started a gaussian calculations for DFT analysis of phytochemical, it is showing a error 2070?

I am doing optimization of a phytochemical but when i submitted the file for calculations it shows error 2070. Can anyone please tell me what does it mean? and what should i do to solve it. I have...

30 January 2021 7,932 1 View

What are the exact phytochemicals present in the plant extracts which act as reducing and stabilizing agents in the green synthesis of Nanoparticles?

There are hundreds of research articles published on the green synthesis of nanoparticles of various metals and metal oxides nanoparticles using plant extract (where mostly the extract of leaves...

30 November 2020 735 6 View

I want to what topic is best for new research from heterocyclic and phytochemicals ?

i am new rearch student i want to know which topic i have to choose for research and why ? i like to chose any heterocylic compound or any of the natural product i-e phytochemicals etc.

04 October 2020 8,299 3 View

What are the best combination of mobile phase used for plant extracts in TLC? what are the techniques used for charecterization of plant extracts?

I want to study the phytochemicals in Petroleum ether, chloroform, and methanol plant extract Please suggest me which solvent system is best for each crude extract. And also suggest techniques...

28 September 2020 8,369 4 View

Why are synthesized compounds like Apigenin not packaged as drugs since they have been identified as having potent pharmacological effects?

These phytochemical compounds have been synthesized and are available in large quantities in commercial labs. Why are they not packaged as finished products for their pharmacological effects?

30 August 2020 5,155 3 View

In phytochemical screening why do we use different solvents?

When we conduct different screening tests to identify phytochemicals in plant extracts, we use more than one solvent. What are the criteria or how do we select the best solvent rather than using...

07 August 2020 2,933 5 View

Any suggestions for phytochemical databases?

I need to find databases for phytochemicals (plants' secondary metabolites) other than Dr. Duke's Phytochemical database and Golm Metabolome Database. Any other suggestions?

08 July 2020 4,205 11 View

Is there any Databases or References to find out which plants contain a specific phytochemical?

Do you know any databases that not only specifies the plant origins of a specific phytochemical, but also demonstrates how much of that substance may be extracted from some specified parts of the...

21 June 2020 6,192 2 View