MINOCYCLINE safe to use 3 months after expiry?

I plan to call my PCP tomorrow to refill it but I had some left over from a spinal infection (which reoccurs) so I took the initial dose today. Its 3 months past its expiry date. I've seen that it...

30 July 2020 9,983 7 View

Why arn't small MW proteins transferring?

25 August 2016 7,374 11 View

Does anyone regularly use a vaporizer to administer isoflurane for anesthetizing mice?

I'm trying to anticipate how much isoflurane I will be using in a year. I will be anesthetizing about 200 mice for about 10 minutes each mouse over the course of a year. Can anyone approximate how...

09 December 2015 2,501 2 View

Why am I getting a peak at the same temperature as my samples for my NEC?

I continually get peaks in my no enzyme control for real time PCR in my melt curve. Though, my Cts are ~7 cycles away. I have DNase treated my RNA twice, changed all reagents and decontaminated...

28 October 2015 6,334 2 View

Would you like to collaborate on a medical device regulatory book contracted through a reputable organization?

03 April 2015 8,554 8 View

Can anyone recommend a general anti fungal drug to add to a bacteria media to avoid contamination?

13 October 2014 152 10 View

Why is the supernatant from my pET expression system lysis step so viscous?

I have been expressing and lysing this construct for years, and today I got a thick gel instead of a nice supernatent. I recently started using Magic Media instead of IPTG induction, but this has...

05 September 2014 5,403 4 View

Which breast cancer cell lines do you find grow best in nude mice?

I have worked exclusively with triple negative MDA-MB-231. We are thinking about redoing projects using a different TNBC cell line or ER+ line such as BT20 or MCF7, respectively. The xenograft...

07 July 2014 8,830 6 View

Domo for decision-making?

I have seen several ads referring to DOMO as a better method for tracking data vs. Excel Spreadsheets because it has the capacity to inform decision-making. Has anyone seen a demo and/or used the...

06 July 2014 7,556 2 View

How long can fresh frozen tissue be stored at -80C?

I need to store fresh frozen tumor tissue from nude mice for western blotting, receptor binding assays, and immunohistochemistry. In our lab, we freeze tissue for western blots in RIPA buffer, in...

14 May 2014 1,481 9 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How can the use of Jolly Phonics improves the quality of reading, speaking and writing in rural disadvantaged communities in Papua New Guinea?

Jolly Phonics reading program is an effective way to teach early reading to young learners. How can lectures at the university prepare early childhood student teachers to teach Jolly Phonics to...

03 March 2021 3,731 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View