How does the bioautography assay of carotenoid (and its constituents) can be carried out to determine the bioactive compound/ spots?

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Can other standards be used to generate IRF file instead of LaB6,Si etc.,,, for Rietveld Refinement?

Hi, my research involves in ZnO synthesis. I have performed rietveld refinement of my samples and obtain a good fit using Fullprof suite software. However to obtain the crystallite size, the IRF...

07 July 2024 3,526 3 View

Can i deduce the presence of surface states based on my XPS data?

In my XPS spectral, i detected oxygen group bonded to carbon and in my zinc spectral (note : my sample is ZnO) ...there are two valent zinc species available, (which is unsual as my previous...

17 June 2024 3,888 2 View

Do your country have Psychology Acts?

Hi/Salam; Did your country establish "Psychology Acts" for psychologists to practice? I just discovered that Malaysia still does not have the Psychology Acts for psychologists to practice, where...

13 May 2024 6,921 6 View

QPCR probe optimization troubleshoot - big CT value, and no ct value in the qpcr result with WT and homozygous confirmed sample. Valid or error?

Im doing a qpcr for genotyping ERCC1 rs11615, now Im in a middle of optimization of Probe ERCC1 wild type, with 2 confirmed sample (WT and heterozygous sample). and the result, Ive got a big CT...

22 November 2023 9,976 4 View

Is it considered bioethics?

If I am working on dealing with sadness as being stigmatized as "sinful/immoral" culturally in Muslim societies that made a roadblock to the process of sad emotion regulation psychologically,...

27 July 2023 9,773 6 View

Are you among all who ‘have a passion for the brain’ as curious humans? Why brain is important to you, your religion, country and your planet Earth?

Join, and show advocacy to the European Brain Council (EBC) which comprises nine member organizations, which are two of them are patient groups, and the other seven groups represent the research...

04 July 2023 8,627 31 View

Why aluminium in aluminium-ion batteries as an anode?

In recent days, batteries have a lot of attention, especially for Electric vehicles. Besides Li-ion Battery, I've read about aluminium ion batteries, but I can't understand, Why aluminium act as...

16 February 2023 6,493 3 View

If I have a sample (corned beef) of 10 g and the maximum nitrite it contains is 30 mg/kg, what is the maximum nitrite in µM? And how to convert it?

Hello, I wanna ask, If I have a sample (corned beef) of 10 g and the maximum nitrite (NO2-) it contains is 30 mg/kg, what is the maximum nitrite in µM? And how to convert it? The research I am...

21 March 2022 6,510 6 View

Is there any hybridized method between the Qur'anic Thematic Analysis and Psychology Thematic Analysis Methods?

Is there any hybridized method between the Qur'anic Thematic Analysis and Psychology Thematic Analysis already developed to refer to in order to complement each others' absent aspect in Islamic...

07 August 2021 9,149 0 View

How to differentiate the product of corrosion and scaling?

I am analyzing the formation of scaling on a geothermal system. The deposit I obtained was from a demister, one of the components there that separated the working fluid from water. I am analyzing...

23 December 2016 2,700 5 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

Controlling for pupil light reflex when analyzing pupil size time course?

I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...

10 August 2024 3,229 0 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Are air moisture harvesting technologies effective in combating desertification?

Air moisture harvesting Air water collection devices

06 August 2024 5,473 2 View

How can I measure the thickness of thin film by Uv-Vis?

06 August 2024 7,810 3 View

Why is TLC not a form of partition chromatography?

Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?

03 August 2024 7,035 3 View

Is there any Indian Company or Startups, from any universiy or IITS, which manufacture Gas Chromatography instrument , i want to make a custOmized G?

or we can collaborate

01 August 2024 6,705 2 View

Z-average size from Dynamic Light scattering can it be lower than the original probe size?

Hi everyone, Recently I have been conducting Dynamic Light scattering experiments in a micellar solution at 5 and gel at 37 degrees of Celsius with latex particles of diameter 190-500 nm. While...

01 August 2024 1,168 4 View

Sylvester Peter Antai

TLC and PC are used for bioautography, but the detection method can be sucessfully improved by the application of advance chromatographic tool namely high performance thin layer chromatography ( HPTLC ), over pressuried layer chromatography ( OPLC ) and planar electro chromatography ( PLC ) .....The zones of the zones of inhibition corresponding to the spots in chromatographic plate are indicative of the substance in question. For more details consult https://www.ncbi.nlm.nih.gov

Salwa Bouabdallah

HPLC Finger print!

Gunawan Indrayanto

Hi, you can check in TLC Camag Data base, Kind regards

https://www.camag.com/en/tlc_hptlc/customer_magazine_cbs/ccbs_database.cfm?method=query