Does cardiac puncture damage the heart for protein analysis or histological staining?

Hello, we have been sacrificing mice and rats using cardiac puncture. I am curious if anyone knows of publications or has had personal experience relating to potentially altered protein...

31 July 2020 681 2 View

Survey for a scientific research in transportation engineering please help?

11 March 2020 4,167 7 View

Is sonicating cells for western blot necessary?

Once cells have been collected in RIPA, before centrifugation (16,000g for 20 min) should cells be sonicated? Does sonication improve protein yield?

14 May 2019 2,385 7 View

What is the purpose of heating RNA at 55-60 C when utilizing the trizol protocol during the last step when RNA is in water?

Does heating influence RNA quality? My understanding was the RNA should not be heated and kept on ice. Trizol protocol:...

13 January 2019 7,933 4 View

Does protein concentration change between freeze and rethaw for western blot??

Hello, My understanding is that protein from cell and tissue lysates degrade between each rethaw. Has anyone experienced differing protein concentrations, significant enough to affect loading...

09 December 2018 6,962 4 View

Hi. Does anybody know an easy way to build the complete sequence of a gene from RNAseq data?

I am trying to identify the unknown sequence of an gene from a plant species which has published RNAseq data deposited to SAR-NCBI. As RNA seq data are all short read data, i am looking for an...

24 February 2018 1,238 2 View

Hi. Does anybody know an easy way to build the complete sequence of a gene from RNAseq data?

I am trying to identify the unknown sequence of an gene from a plant species which has published RNAseq data deposited to SAR-NCBI. As RNA seq data are all short read data, i am looking for an...

01 February 2018 4,649 2 View

How I can relate the loading frequency to the operational vehicle speed in the dynamic modulus of elasticity of asphalt pavement?

28 February 2017 7,686 2 View

Is there tables to get trip generation rates for marine yard in Gulf area?

12 April 2016 980 2 View

How can I optimize the procedure to determine platelet CD markers expression by flow cytometry?

Greetings, Could you please clarify these points to me. I have a study on platelet CD markers expression by flowcytometrey: -If I prepared platelet rich plasma without fixing the cell # and...

12 December 2015 8,820 13 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View