How does SnapGene decide where open reading frames start?

20 July 2020 1 5K Report

When I click the yellow and green arrow icon, and select "ORFs only", how does the software decide where to start ORFs (since it is not 100% dependent on the starting methionine)?

Ghufran Aljuburi

Good question

Badges
Science topic

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

What plants have high levels of sulfur-containing amino acids such as methionine?

Methionine, cysteine, homocysteine, and taurine are the 4 common sulfur-containing amino acids, but only the first 2 are incorporated into proteins. Sulfur belongs to the same group in the...

22 July 2024 4,273 4 View

How to quantify the fluorescence intensity of only the merge area of an image using ImageJ software?

Dear All: I have to quantify the fluorescence intensity of cells that are fluorescent to two different markers, so i'm interested in finding out if this is possible. Previously I have...

22 July 2024 8,770 1 View

What are the key risks associated with foodborne pathogens in imported leafy greens, the impact on public health and effect on regulatory framework?

I am currently writing a systematic review

15 July 2024 9,176 1 View

How do i go about my research which is about the integration of solar and wind power to produce green hydrogen?

Let me say I have a solar plant in operation and I want to build up a wind power plant, and later make a hybrid so that I could power the electrolyser as load for green hydrogen production.

14 July 2024 9,547 4 View

I need to connect expert in Urban design for Health ?

I studying PhD course in Urban design for Health. I need to know in some fileds: residental block, green open space and Transport TOD. I need connect with expert in that fileds.

14 July 2024 1,892 3 View

When Using SYBR Green for Flourescent Detection of LAMP Products what causes an Overshoot in Flourescence Reading?

The Graph below shows an example of an amplification curve that shows an overshoot in fluorescence. I am using SYBR Green, BST polymerase, and 6 LAMP primers in the mixture and amplifying at 65...

14 July 2024 1,483 0 View