How does one separate nanopartcles from bacterial biomass?

More Mansoure Hosseini's questions See All

About loading of Doxorobicin on MOF (drug delivery)?

Dear Researcher Respectfully, I am working on drug delivery. I synthesized Zn-MOF. I am going to load Doxorobicin on it and the study the derivery behaviour. I tried several times to load. but I...

07 February 2021 748 2 View

How can I represent a 2-parameters function in series type?

I want to represent a function like f(x,y)=sin(1/(x+y)) about [x,y]=[0,0] in series type. how can i do it in matlab or maple?

10 January 2021 8,389 5 View

Can you recommend a protocol for immunohistochemistry of mouse brain sections using anti-MAP2?

Current protocol does not give clear images.

04 January 2021 1,614 3 View

Challenges in growing deep learning model?

I implemented an image classifier and object detection model. I added a new class every day to my model and the data set growing too. I wanted to ask if anyone has the same experience? at now it...

03 January 2021 4,652 5 View

Anyone knows how to extract RNA from samples with high starch content (wheat) by using RNeasy Plant Minikit?

I am using 74904 to extract RNA from wheat seed and I have tried both of the lysis buffer. As it is mentioned it protocol, buffer RLT is the lysis buffer of choice but it may cause...

14 October 2020 9,409 7 View

What causes different microstructure in a sample?

I have synthesized high entropy oxide with (Ni Zn Cu Mn)1-x Cox composition. At x=0.05 the microstructure became rod shape and the SEM picture of the other part of that sample is different. And...

12 October 2020 5,576 5 View

How to increase zebrafish survival rate after spinal cord injury ?

Hi everyone I'm having trouble with the high mortality rate of my zebrafish after surgery. ( the surgery is done based on this article: Becker, C. G., Lieberoth, B. C., Morellini, F., Feldner,...

13 September 2020 6,781 1 View

Antibiotic screening of drug resistance cancer cell line with MDR pumps?

Hi. during our study, we faced with a problem in the screening of EPG RDB 257 cell line which is a resistant cell line due to the expression of efflux pumps such as MDR 1 which actively excrete...

27 August 2020 9,192 1 View

How to work with IQF device with the highest efficiency?

Hello I work with the IQF machine My problem is that when wet vegetables enter the machine, the efficiency of the machine decreases. What can be done to remove moisture from vegetables without...

01 August 2020 8,179 1 View

In response surface methodology, can we use sum of the response signals for two dependent variables as a function of independent variables?

RSM based on CCD is supposed to be applied for finding the optimum value of some independent variables. There are two dependent variables. Can we use sum of the response signals obtained for these...

04 July 2020 4,865 7 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

How can I calculate the rdf of water molecules around 'Na' ions that are closest(adsorbed) to the nanoparticle(solute)?

Hi, I want to calculate the radial distribution function for water molecules around the Na+ ions adsorbed on the surface of a spherical nanoparticle using the gromacs trajectory dynamically? For...

28 February 2021 5,650 1 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

Marité Cárdenas

I guess that you can use a centrifugation gradient?

Mansoure Hosseini

Thank you.

In new publication have been used centrifugation and has been successful.

Matteo Ardini

Hi!

Use ultracentifugation (20000 rpm, 30 min) as suggested by Marité to remove cells. After use again centrifugation (3000 rpm, 30 min) with 30 kDa or less centricon devices to separate NPs from proteins/DNA (NPs should go into the flow-through if they are 50 nm or less). I hope this will be useful!