Hi Bipinraj,

try ploylysinated slides to immobilize that should work.

see e.g. M&M: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC15048/

Hi Bipinraj,

that depends on what you would like to do.  For instance, if you want to determine them alive to see apoptotic corpses, then you could prepare 2% Agar pads. We normally do this for DIC microscopy. It is really fast and cheap. Just prepare the 2 % Agar with  M9 buffer and pipette ~500 ul on a slide. Put a second slide on top to squeeze the agar. In order to immobilize the worms one can use 5 ul Sodiumazide solution (30 nM). You could also buy DAPI containing Fluoromount G solution (SouthernBiotech) when using dead worms in immunostainings.

Dear Ingo Fricke

Thank you very much for the link.

Dear Michael Schell ·

Thanks for the method. It was very useful

  if you want to see the worm alive it can be mounted on a cavity slide or  microwell plate or small petri dish.  . To immobilise the worm slighty warm the slide or plate by keeping it an incubator at 40-45 degree centigrade for a few minutes.