We want to study some antioxidants and assess their relative effectiveness. However we wish to induce oxidative stress preferably using dietary additives.
Dear Akalpita,
The attached publications demonstrate the effect of inducing oxidative stress (experimentally) on rats:
1-EPMA J. 2014; 5(1): 2.
Published online 2014 Jan 13. doi: 10.1186/1878-5085-5-2
PMCID: PMC3922789
The efficacy of probiotics for monosodium glutamate-induced obesity: dietology concerns and opportunities for prevention
Oleksandr A Savcheniuk,1 Oleksandr V Virchenko,1 Tetyana M Falalyeyeva,1 Tetyana V Beregova,1 Lidia P Babenko,2 Liudmyla M Lazarenko,2 Olga M Demchenko,3 Rostyslav V Bubnov,corresponding author2,4 and Mykola Ya Spivak
Abstract
Introduction
Obesity becomes endemic today. Monosodium glutamate was proved as obesogenic food additive. Probiotics are discussed to impact on obesity development.
Aims and objectives
The aim was to study the effects of probiotics on the development of monosodium glutamate (MSG)-induced obesity in rats.
Material and methods
We included 45 Wistar male rats and divided into three groups (n = 15). Newborn rats of group 1 (control) received subcutaneously 8 μl/g saline. Group 2 received 3 to 4 mg/g MSG subcutaneously on the second, fourth, sixth, eighth and tenth day of life. Within 4 months after birth, rats were on a standard diet. Group 3 received an aqueous solution of probiotics mixture (2:1:1 Lactobacillus casei IMVB-7280, Bifidobacterium animalis VKL, B. animalis VKB) at the dose of 5 × 109 CFU/kg (50 mg/kg) intragastrically. Administration of probiotics was started at the age of 4 weeks just after weaning and continued for 3 months during 2-week courses. Group 2 received intragastrically 2.5 ml/kg water. Organometric and biochemical parameters in all groups of rats were analyzed over 4 months. The concentration of adiponectin was determined in serum, and leptin - in adipose tissue.
Results
Administration of MSG led to the development of obesity in rats; body weight had increased by 7.9% vs controls (p < 0.05); body length had increased by 5.4% (p < 0.05). Body mass index and Lee index and visceral fat mass had increased (p < 0.001). Under the neonatal injection of MSG, the concentration of total cholesterol, triglycerides, VLDL cholesterol and LDL cholesterol significantly increased (p < 0.001), in comparison with controls. Adipose-derived hormones changed in MSG obesity rats: adiponectin decreased by 58.8% (p < 0.01), and leptin concentration in adipose tissue had increased by 74.7% (p < 0.01). The probiotic therapy of rats from group 3 prevented obesity development. Parameters of rats treated with probiotic mixture did not differ from that in the control.
Conclusions
The introduction of MSG to newborn rats caused the obesity in adulthood. Periodic administration of probiotic mixture to rat injected with MSG neonatally resulted in recovery of lipid metabolism and prevention of the obesity development.
Keywords: Predictive, preventive, personalized medicine, obesity, probiotics, animal model, monosodium glutamate, food additives, personalized dietology
2-J. Rad. Res. Appl. Sci., Vol.4, No.1(B), pp. 301-316 (2011)
Beneficial use of continuous administration of crataegus
oxycantha in irradiated male albino rats
M. I. Michael
ABSTRACT
Exposure to ionizing radiation has become frequent with its vast applications in
diagnosis, treatment and industry rather than possible environmental pollution
with radiation. The animals of this study were allocated into three groups,
control group, irradiated group (5 Gy one shot) and hawthorn treated postirradiation
group. Exposure to radiation was implicated with hematological
suppression in all hematological parameters tested red blood cells count
(RBCs), hemoglobin content (Hb), hematocrit percentage (HCT) and white
blood cells count (WBCs). Radiation exposure caused alteration of lipid
metabolism leading to increased total lipids, triglycerides, total cholesterol, and
low density lipoprotein (LDL) and decreased high density lipoprotein (HDL). In
addition, liver enzymes were also elevated, while serum albumin was
diminished referring to liver dysfunction. The male reproductive hormone
(testosterone) was severely inhibited along with glutathione. Administration of
extraction of Crategous oxinatha (hawthorn) with a dose of 500 g/kg b.w. orally
for consecutive 15 days post-irradiation showed significant ameliorating effects
on both hematological and biochemical parameters leading to the suggestion of
using hawthorn as food additive in relatively high amount.
Keywords: Radiation, Hawthorn, Hematology, Lipid profile, Liver enzymes,
Testosterone, Glutathione.
3- itation: Clinical and Translational Gastroenterology (2013) 4, e39; doi:10.1038/ctg.2013.11
Published online 11 July 2013
Functional GI Disorders
The Effect of High-Fat Diet-Induced Pathophysiological Changes in the Gut on Obesity: What Should be the Ideal Treatment?
Open
Chooi Y Lee PhD1
Abstract
Obesity is a metabolic disorder and fundamental cause of other fatal diseases including atherosclerosis and cancer. One of the main factor that contributes to the development of obesity is high-fat (HF) consumption. Lipid ingestion will initiate from the gut feedback mechanisms to regulate glucose and lipid metabolisms. But these lipid-sensing pathways are impaired in HF-induced insulin resistance, resulting in hyperglycemia. Besides that, duodenal lipid activates mucosal mast cells, leading to the disruption of the intestinal tight junction. Lipopolysaccharide that is co-transited with dietary fat postprandially, promotes the release of cytokines and the development of metabolic syndrome. HF-diet also alters microbiota composition and enhances fat storage. Although gut is protected by immune system and contains high level of antioxidants, obesity developed presumably when this protective mechanism is compromised by the presence of excessive fat. Several therapeutic approaches targeting different pathways have been proposed. There may be no one single most effective treatment, but all aimed to prevent obesity. This review will elaborate on the physiological and molecular changes in the gut that lead to obesity, and will provide a summary of potential treatments to manage these pathophysiological changes.
Hoping this will be helpful,
Rafik
Dear Akalpita,
The attached publications demonstrate the effect of inducing oxidative stress (experimentally) on rats:
1-EPMA J. 2014; 5(1): 2.
Published online 2014 Jan 13. doi: 10.1186/1878-5085-5-2
PMCID: PMC3922789
The efficacy of probiotics for monosodium glutamate-induced obesity: dietology concerns and opportunities for prevention
Oleksandr A Savcheniuk,1 Oleksandr V Virchenko,1 Tetyana M Falalyeyeva,1 Tetyana V Beregova,1 Lidia P Babenko,2 Liudmyla M Lazarenko,2 Olga M Demchenko,3 Rostyslav V Bubnov,corresponding author2,4 and Mykola Ya Spivak
Abstract
Introduction
Obesity becomes endemic today. Monosodium glutamate was proved as obesogenic food additive. Probiotics are discussed to impact on obesity development.
Aims and objectives
The aim was to study the effects of probiotics on the development of monosodium glutamate (MSG)-induced obesity in rats.
Material and methods
We included 45 Wistar male rats and divided into three groups (n = 15). Newborn rats of group 1 (control) received subcutaneously 8 μl/g saline. Group 2 received 3 to 4 mg/g MSG subcutaneously on the second, fourth, sixth, eighth and tenth day of life. Within 4 months after birth, rats were on a standard diet. Group 3 received an aqueous solution of probiotics mixture (2:1:1 Lactobacillus casei IMVB-7280, Bifidobacterium animalis VKL, B. animalis VKB) at the dose of 5 × 109 CFU/kg (50 mg/kg) intragastrically. Administration of probiotics was started at the age of 4 weeks just after weaning and continued for 3 months during 2-week courses. Group 2 received intragastrically 2.5 ml/kg water. Organometric and biochemical parameters in all groups of rats were analyzed over 4 months. The concentration of adiponectin was determined in serum, and leptin - in adipose tissue.
Results
Administration of MSG led to the development of obesity in rats; body weight had increased by 7.9% vs controls (p < 0.05); body length had increased by 5.4% (p < 0.05). Body mass index and Lee index and visceral fat mass had increased (p < 0.001). Under the neonatal injection of MSG, the concentration of total cholesterol, triglycerides, VLDL cholesterol and LDL cholesterol significantly increased (p < 0.001), in comparison with controls. Adipose-derived hormones changed in MSG obesity rats: adiponectin decreased by 58.8% (p < 0.01), and leptin concentration in adipose tissue had increased by 74.7% (p < 0.01). The probiotic therapy of rats from group 3 prevented obesity development. Parameters of rats treated with probiotic mixture did not differ from that in the control.
Conclusions
The introduction of MSG to newborn rats caused the obesity in adulthood. Periodic administration of probiotic mixture to rat injected with MSG neonatally resulted in recovery of lipid metabolism and prevention of the obesity development.
Keywords: Predictive, preventive, personalized medicine, obesity, probiotics, animal model, monosodium glutamate, food additives, personalized dietology
2-J. Rad. Res. Appl. Sci., Vol.4, No.1(B), pp. 301-316 (2011)
Beneficial use of continuous administration of crataegus
oxycantha in irradiated male albino rats
M. I. Michael
ABSTRACT
Exposure to ionizing radiation has become frequent with its vast applications in
diagnosis, treatment and industry rather than possible environmental pollution
with radiation. The animals of this study were allocated into three groups,
control group, irradiated group (5 Gy one shot) and hawthorn treated postirradiation
group. Exposure to radiation was implicated with hematological
suppression in all hematological parameters tested red blood cells count
(RBCs), hemoglobin content (Hb), hematocrit percentage (HCT) and white
blood cells count (WBCs). Radiation exposure caused alteration of lipid
metabolism leading to increased total lipids, triglycerides, total cholesterol, and
low density lipoprotein (LDL) and decreased high density lipoprotein (HDL). In
addition, liver enzymes were also elevated, while serum albumin was
diminished referring to liver dysfunction. The male reproductive hormone
(testosterone) was severely inhibited along with glutathione. Administration of
extraction of Crategous oxinatha (hawthorn) with a dose of 500 g/kg b.w. orally
for consecutive 15 days post-irradiation showed significant ameliorating effects
on both hematological and biochemical parameters leading to the suggestion of
using hawthorn as food additive in relatively high amount.
Keywords: Radiation, Hawthorn, Hematology, Lipid profile, Liver enzymes,
Testosterone, Glutathione.
3- itation: Clinical and Translational Gastroenterology (2013) 4, e39; doi:10.1038/ctg.2013.11
Published online 11 July 2013
Functional GI Disorders
The Effect of High-Fat Diet-Induced Pathophysiological Changes in the Gut on Obesity: What Should be the Ideal Treatment?
Open
Chooi Y Lee PhD1
Abstract
Obesity is a metabolic disorder and fundamental cause of other fatal diseases including atherosclerosis and cancer. One of the main factor that contributes to the development of obesity is high-fat (HF) consumption. Lipid ingestion will initiate from the gut feedback mechanisms to regulate glucose and lipid metabolisms. But these lipid-sensing pathways are impaired in HF-induced insulin resistance, resulting in hyperglycemia. Besides that, duodenal lipid activates mucosal mast cells, leading to the disruption of the intestinal tight junction. Lipopolysaccharide that is co-transited with dietary fat postprandially, promotes the release of cytokines and the development of metabolic syndrome. HF-diet also alters microbiota composition and enhances fat storage. Although gut is protected by immune system and contains high level of antioxidants, obesity developed presumably when this protective mechanism is compromised by the presence of excessive fat. Several therapeutic approaches targeting different pathways have been proposed. There may be no one single most effective treatment, but all aimed to prevent obesity. This review will elaborate on the physiological and molecular changes in the gut that lead to obesity, and will provide a summary of potential treatments to manage these pathophysiological changes.
Hoping this will be helpful,
Rafik
Thanks Rafik for the answer. I will investigate the third option. But basically these and other compounds may induce other pathways too. We are looking for some more specific oxidative stress inducers. Doxorubicin was also suggested to us as a useful inducer. Has anyone used this compound for induction of oxidative stress?
Dear Akalpita Arvindekar:
If you are looking for oxidative inducer for animal model, according to the previous study of our lab, I will recommend you "D-galactose".
I had attached the publication bellow.
hope it will be useful!
Article Antioxidant activities of chicken liver hydrolysates by peps...
I will suggest you to consider Rotenone which block electron transport chain that leads to increased superoxide leakage from mitochondria.
Dear Dr. Ru-Jeng,
Have you tried using rotenone? If so what should be the dose? Being an ETC inhibitor will it not lead to death of the animal?
Best regards
- Badges
- Science topic