How does one go about detecting/identifying bacteriophages in mixed samples?

More Shahin Khazai's questions See All

How do i analyze scratch assay data obtained thru imageJ?

hello. i manually measured the area in each image. the output i got was area/mean/min/max then i put all the data into excel, and also added the following columns: group names/date/hour pic was...

19 January 2021 3,936 3 View

What are the alternative to Rotary Evaporation?

I want to remove solvent to obtain a solid . i would like to remove solvents such as THF, TEA, carbon tetrachloride . the obtained solid which is a Phosphorus ester. What could be an suitable...

04 January 2021 1,220 1 View

ENVI error when opening Metadata?

ENVI software (V 5.3) has a problem with opening some Metadata files. An error appears which means it does not seem to be a Metadata file. How can I solve this problem?

20 December 2020 4,590 3 View

How to detect saturated pixels in Landsat images?

I have heard pixels with DN>10000 in Sentinel-2 images are saturated pixels. How to detect saturated pixels by DN in Landsat images? Is there any reference that has determined these DN numbers for...

16 December 2020 5,827 3 View

What is the role of Phytomelanin in seed coat?

We know commonly found in seed coat at Asparagales and Asteraceae family But, what is the role of Phytomelanin in the seed coat? Is it only a protector?

03 December 2020 7,493 5 View

Do NPC or FC structures have power loss of capacitor ripple?

I should say that when capacitors are parallel with input voltage DC, they have capacitor ripple voltage loss cause of direct connection but when a capacitor is in path of charging current -it's...

04 September 2020 9,774 1 View

In gas chromatograpy, how do I propagate SD of 5 readings at each standard conc. along with the SE of the coefficients in a quadratic regression?

Hi, This question pertains to gas chromatography to measure levels of carbon monoxide using reduction gas detector technology. I have developed a quadratic regression using 6 standard CO...

11 April 2019 7,888 3 View

I have a check box question, where the respondents can choose more than one choice, how can I insert more than one choice in the SPSS?

I have a check box question, where the respondents can choose more than one choice, how can I insert more than one choice in the SPSS extraction sheet? e.g: 1. medical 2. surgical 3....

27 February 2019 4,397 3 View

Nuclear extraction from brain tissue?

Hi, I'm looking for an inexpensive and efficient method to isolate nuclear fraction from brain tissue. There are kits and protocols available, but they are mostly for cells and tissues other than...

14 January 2019 402 2 View

What are the basic characteristics of Big Data?

I collected more than 200000 samples, but someone said the data was not a Big data. I asked him to define the Big data, unfortunately he could not define.

15 August 2018 4,985 12 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Why is the sum of the elements in a row in a Q matrix equal to zero?

When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?

28 February 2021 3,864 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

How to improve and implement WBAN MAC and Radio modules under Castalia 3.2 ?

Hello researchers, Hello Castalia3.2 researchers I am a beginner with Castalia, I work on optimization of RFID systems used in wireless body networks. I simulated a WBAN with 10 nodes and one...

28 February 2021 400 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Henrik Rasmus Andersen

If you are looking more generally for phages and have many environmental samples, DNA extraction and sequencing makes more sense.

DNA extraction and sequencing does identify the phages and it can cope with many phages in one sample. Additionally one can process many samples per day.

How long does it take to prepare a sample by negative staining and identify 100 phages by TEM?