Hello,
I have recently started reading about fermentation and inevitably stumbled across the description of the composition of the culture medium adapted to a given host organism.
Here is a typical example from my reading of the article Article Pichia pastoris versus Saccharomyces cerevisiae: a case stud...
."Recombinant P. pastoris X33::hgm-csf was grown in 10 mL BMGY medium (2% peptone, 1% yeast extract, 0.34% yeast nitrogen base, 1% ammonium sulfate, 1% glycerol, and 0.4 mg/L biotin, buffered with 1/10 volume of pH 6.0 potassium phosphate buffer) at 30°C with constant shaking at 250 rpm until the culture reached an OD600 of 2–4. The cells were harvested and re-suspended in 10 mL BMMY (0.5% methanol is substituted for 1% glycerol in BMGY) with the same growth conditions. For induction, methanol was added every 24 h to a final concentration of 0.5%. After 72 h, culture supernatants were collected by centrifugation for further analysis."
I wonder how one determines the appropriate concentrations of each of the components of the medium ? Are there some key findings from Medium Optimization research that one can use to design optimal medium ? Or is the current state-of-the-art research still lacking clear models and experiments are the only way to go ?
Thanks,
Denis Joly
- Badges
- Science topic
- Similar topics
- Gels