for oxalate, i used 1g sample, 75ml 0f 3M H2SO4 to soak it. filtered and took 25ml aliquot and warm and titrate against 0,05M KMnO4. (1ml KMnO4=2.2mg).
for phytate, i used 2g sample, soak with 100ml 0f 2%HCl, filter and took 25ml aliquot and to it, 53.5ml dH20 was added. 10ml 0.3% ammonium thiocyanate soln was added and titrated against standard iron III chloride containing 0.00195n iron/ml.
how do i determine these values in mg/100g?
thanks. swift response is anticipated
Amina Benabbou
Here a study that used the same method you sited, may be it's useful for you.
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Franklin Ohikhena
thanks Amina but the method didnt state how they were calculated. i need detailed steps of the calculations
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Camelia Maier
You can estimate spectrophotometrically the amount of oxalate in your samples by using an oxalate standard curve. You can then express it as mg/100g.
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