How does Nile Red specifically stain PHB in microalgae?

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Quantification of CD8 positive cells using ImageJ software?

Hello everyone, I am working with mammary fat tumors from mice. My goal is to characterize tumor-infiltrating immune cells between different tx groups. I did immunofluorescence staining with...

01 July 2024 8,964 1 View

Can we use different Likert point scale for SEM-PLS ?

We have two sets of data, and we want to conduct SEM-PLS and multi-group analysis via SmartPls. However, one set of data is 5 point Likert scale while another is 7 point Likert scale. Do I need to...

21 May 2024 8,548 4 View

Quadratic Mixed Model Assumptions?

Hello, I am new to mixed effects model and am running a analysis with it. However, am wondering if the assumptions check for Quadratic mixed models are the same as for linear mixed model? -...

15 May 2024 7,716 3 View

Help me to find XRD jcpds card information?

I would be extremely grateful if someone could help me find the following cards: CaZnOS PDF-01-072-3547 SrZnOS PDF card # 041-0551 BaZnOS PDF #17-1239 Ba0.5Na0.5TiO3 - SrTiO3 Thanks a lot!

08 May 2024 5,034 1 View

How to protect human DNA during centrifugation?

I have been trying to collect bacteria from human fecal sample to run on MT-PCR, however, for some reason, the concentration of human DNA is quite low and could not confirmed on PCR. My technique...

07 May 2024 8,173 1 View

What is the meaning of depth and level in Imaris statistic analysis (filament function)?

I used filament tracing of Imaris to reconstruct dendritic spine and I got the statistic result of dendritic spine density. I wanted to count dendritic spine density in secondary and tertiary...

06 May 2024 8,675 0 View

Why protein bands are unclear or invisible on SDS PAGE gel after staining?

Hi everyone! I have carried out SDS PAGE analysis many times. In the experiment, protein sample was hydrolyzed gelatin. After staining, only bands of protein marker (standard) were shown, whereas...

24 April 2024 6,444 1 View

Hello! I hope you're well. How can I establish the molecular equation of a biomass sample? Any recommended techniques?

I utilize a photosynthesis reactor for wastewater treatment. Within the reactor, I collect biomass and aim to establish its molecular equation of biomass, such as CxHyNzPtKi.... I would greatly...

20 April 2024 6,384 6 View

How do I perform data analysis in Excel on two variables with varying amount of questions and values?

I am studying leadership style's impact on job satisfaction. in the data collection instrument, there are 13 questions on leadership style divided into a couple of leadership styles. on the other...

16 April 2024 4,784 3 View

How to Handle Multiple RTF and PRM Files with the charmm2lammps.pl Script?

I have previously used the charmm2lammps tool to generate LAMMPS inputs multiple times, specifically for simple protein systems. For those systems, I was able to create LAMMPS input from a PSF+PDB...

14 April 2024 8,470 0 View

Is there any cases algae not using the nutrient from the wastewater and grow normally?

I am working on microalgae cultivation using waste water. The initial concentration of nutrients were less but the microalgae has achieved biomass growth of 2 g/L. The final concentration of...

08 August 2024 4,812 2 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Suggestions for the Annexin V Positive Control ?

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29 July 2024 8,624 1 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ?

Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...

25 July 2024 7,520 0 View

Samia Muhammad Arif

you can use the Fluorescence Emission Spectra technique, as both PhB and lipids show peaks at different wavelengths. Following reads may be helpful:

Chapter Screening for Lipids from Marine Microalgae Using Nile Red

Article A high throughput Nile red fluorescence method for rapid qua...

Article The use of fluorescent Nile red and BODIPY for lipid measure...

Ka Ho Pak

Thank you!