Which sequence could induce higher immunogenicity, S polypeptide or only S1? and how to isolate S1 from a vector containing the whole seq. of S?

I have a clone of DNA sequence encoding the full spike protein (S1+S2)

01 January 2021 7,821 1 View

Any recommendation for Bacmid DNA isolation?

29 November 2020 5,284 6 View

Why RNA is totally degraded?

18 October 2020 5,252 22 View

How to prepare 37% Formaldehyde from paraformaldehyde?

I want to prepare 37% formaldehyde for denatured formaldehyde agarose gel preparation (for RNA) How can I prepare 37% formaldehyde? And how long this solution would be stable?

11 October 2020 914 4 View

What is the concentration of saturated solution of bromophenol blue?

I want to know the concentration of a home-made saturated aqueous solution of Bromophenol blue. How could I know when I add unknown number of grams of the dye?

05 October 2020 3,423 3 View

What is a good pretext task for self supervised lane detection in autonomous driving?

I have been looking into self-supervised methods in computer vision. For example Digging Into Self-Supervised Monocular Depth Estimation which looks at three consecutive frames of video, tasks a...

23 July 2020 8,232 1 View

Looking for reliable databases for thermophysical data for Phase change material for ML?

Hi everyone, I have been having a bit of a hard time finding data on phase change material, like Paraffin, salt hydrates, fatty acids... on the scale of 100s to 1000s. The big repositories only...

28 June 2020 290 3 View

Does insect such as flies and mosquitoes transmit the COVID-19 virus as a mechanical transmission?

Does insect such as flies and mosquitoes transmit the COVID-19 virus as a mechanical transmission? Virus transmission take place by insect legs, mouth parts and other body parts.

15 June 2020 6,000 98 View

What are the public datasets available for emotion recognition?

I am currently working on emotion recognition. I am looking for a dataset that contains labeled image files for emotion (i.e. happiness, sadness, etc). I've found several dataset but not exactly...

06 May 2020 9,650 7 View

What are some ways (aside from: a. mouse weight b. girth) to measure tumor growth in mice?

We have mice injected w/ specific cancer cell lines. The cancer cell lines failed to pick up the luciferase bioluminescent tag. We have a rubric of categories to measure the mice, but I was...

25 March 2020 5,991 2 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

Phenol-pH graphic?

We want to use phenol pH graphic to measure the pH of mediums before and after inhibitor application to cells. We have phenol absorbence values at 460, 560 and 720 nm wavelength. When we measure...

20 February 2021 7,700 3 View

Which way will be better to give OGT inhibitors doses through intravenous or orally for pregnant female rats and concentration suggestion?

I'm planning to use ST078925 OGT inhibitor from TimTec (OGT-O-linked N-acetylglucosamine (GlcNAc) transferase) for female rats.

16 February 2021 7,850 2 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Has anybody administered MK-2206 (an orally active, allosteric inhibitor of AKT) intravenously to the mice?

We aim to use MK-2206 to inhibit AKT in mice. However, a large body of studies suggests that it should be administered orally. I was wondering if somebody could tell me that is it toxic for the...

07 February 2021 6,340 1 View

ACE2 Blotted as a double band?

Hello, I have recently run a western of Vero cells for ACE2. I detected ACE2 at the correct size, but it presents as a double band. The sample was prepared by lysing cells in 1X Laemmli...

07 February 2021 6,973 3 View

Can any tell me or suggest any research article or review about the concentration of peptide dissolve in system water and given to Adult Zebrafish?

Hello, everyone! I have chemically synthesized antioxidant peptide isolated from the plant. I have to validate its bioactivities in Adult zebrafish. I have to administer this peptide to zebra...

01 February 2021 5,809 3 View

Sample Size for Quasi-Experimental Quants using one-tailed t-test?

Hi, sorry I'm stumped. I'm going to do a study on the leadership styles (using the MLQ Form 5X) of minority women administrators in medical schools. How will I calculate the sample size? Thank...

30 January 2021 2,865 4 View