Phytoremediation and contaminated land
I am planning my protocol for my dissertation for an experiment quantifying bacterial (E. coli) biofilm formation on micro-plastic (5mm discs of polyethylene bags) by conducting a crystal violet...
23 February 2021 7,868 2 View
Hi all! My work team and I are looking for papers on muscular descriptions of the hindlimbs of birds (extant and extinct) that include the intrinsic muscles of digits. Any help will be more than...
22 February 2021 8,330 6 View
Currently, I'm working with banana fruits. I've tried to do RNA extractions with different methods, and I've gotten total RNA without contamination, but I couldn't get a high concentration of...
22 February 2021 6,126 2 View
Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...
21 February 2021 7,260 4 View
Hi, I am experiencing some difficulties with 3T3-L1 cells culturing recently. I am working with 3T3-L1 cells for almost 4 years now and have seen bacterial contaminations/ unhealthy cells and...
13 February 2021 4,040 3 View
I am working on a biosensing project on paper substrate, however, it is found that the RNase contamination occurs on the filter paper. Might I ask is there any method to clean the paper surface...
12 February 2021 3,926 3 View
I'm looking to try this in place of normal goat serum for a wisteria floribunda lectin stain. In my IF protocol, 10% of the final volume of my blocking solution is goat serum. If I were to go the...
07 February 2021 7,520 6 View
Hello! Our lab is working with primary cells which we are isolating from the colorectal tissue. During the isolation, we are washing the tissue with pen-strep and then also add some pen-strep in...
07 February 2021 8,258 3 View
Hi everyone, I have been culturing HEK293T cells that have been engineered to overexpress the mechanosensitive protein Piezo1. However, I am finding it very difficult to keep them in culture -...
02 February 2021 7,892 4 View
Engine oil extraction from contaminated soil,10kg of contaminated soil+20ml of dicholoromethane+? of anhydrous sulphate
01 February 2021 9,509 1 View
