How can we limit artifacts caused by rotation in fiber photometry?

We are trying to run fiber photometry experiments for long time scales, but we get these sinusoidal artifacts that come up. We think they may be caused by twisting of the fiber optic subject...

25 February 2021 568 1 View

Which one is better for storing energy: Fuels synthesized from CO2 or Hydrogen?

Based on educated guesses, which one of the following promises a better future for storing energy for grid scale, automobiles etc as opposed to batteries: 1) Fuels synthesized form CO2 2)...

02 February 2021 8,741 6 View

What is the best way to create a Vector file for any figure?

Some journals ask for a vector file for the figures/diagrams submitted for the article. What is the best way to create a vector file from your Microsoft Excel chart?

01 February 2021 6,344 2 View

Aluminium has quality standards of scraches and cupping etc?

Our company made railway car body with AL. And there are some scratches and cupping. I don't know whether there is a quality standard of it or not. ex) Depths of scratches have to be less than...

12 August 2020 4,498 2 View

How can I simulate a DOS attack in 5G?

I have been using NetSim simulator and now I want to try to simulate a DOS attack in 5G. Can someone guide me on the best way to implement this in NetSim?

27 May 2020 9,187 6 View

What are your views about the flooded AI research in the community?

The current overwhelming AI research reminds me the wave of nano research during 1990s. Too many nano researchers could not find jobs. Currently, many nano researchers are changing their research...

19 March 2020 3,173 14 View

Does anybody provides identification of avian nematodes in India?

Nematodes were found in some birds during the postmortem. I would appreciate if somebody expert in nematode taxonomy would help in the identification ...

30 January 2020 7,758 3 View

How Can I do Natural Convection?

In my model I'm doing plastic extrusion simulation with motion of nozzle simultaneously. So material is flowing continuously and when material comes out of nozzle I want to add convection and...

15 August 2019 8,685 4 View

Does anyone know of an instrument that can be used to test the number of Behavioral Addictions (BA) in an individual?

The Shorter PROMIS Questionnaire (SPQ) would have been perfect for my research, but I cannot seem to find a way to contact anyone who could approve the use of such instrument. I am looking for an...

18 February 2019 2,070 3 View

How to prepare deoxyhemoglobin by nitrogen bubbling?

I prepared deoxyhemoglobin by mixing a few drops of Stokes' reagent into a certain concentration of oxyhemoglobin solution. The deoxyHb was confirmed by UV-vis spectroscopy. I also tried to use...

20 December 2018 6,221 7 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View