27 October 2016 1 3K Report

Is there a macula and fovea? 

What is the vascular pattern in their retinas? 

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How to passage and maintain healthy human astrocytes?

Hi all, I have bought a line of human glia (astrocytes) and want co-culture it with cortical neurons differentiated from iPSCs. I want to maintain these cells and passage them for future use, I...

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How to cryopreserve healthy human astrocytes?

I have a line of healthy human astrocytes, and plan to passage it. I was wondering if any suggestions on how to cryopreserve it? I use mFreSR from stem cell to freeze the iPSCs and not sure if I...

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Has anyone ever kept the whole Matrigel bottle in 4C instead of making aliquots?

Hi all, Here is a simple technical question: I have been making aliquots of Matrigel and saving them for each experiment. However, an expert in our lab, who has now left, showed me a few weeks...

03 May 2021 3,680 0 View

Black dots and round shaped structures formed on iPSC culture, is it contamination?

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10 March 2021 6,297 6 View

What were the conditions for the teaching of linear perspective techniques in the Renaissance?

Was training the principles of linear perspective accessible to everyone? Please recommend articles, books and any research that clarifies the conditions for perspective teaching in the workshops...

30 March 2020 8,259 3 View

Explain the primer dimer phenomena in PCR results?

the band in gel electrophoresis appear under the ladder

11 December 2018 8,717 7 View

What the differences between direct and in direct ELISA?

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Ni-NTA purification?

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Generating GAG affinity matrix for protein purification.

Does anyone have experience attaching glycosaminoglycans (CS, KS, HA, HS) to GE Healthcare Epoxy-activated Sepharose 6B beads? Thanks.

24 June 2013 9,520 1 View

What are the uses of antibiotic in livestock industry

antibiotic used in cattle and poultry farms for many reasons such as increase growth, prevention and treatment On the other hand, antibiotic usage in livestock animals have negative effect for...

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