Has anyone ever handled Liberase TL? I dissolved enzyme powder and aliquot. After that, I quick-froze and stored them in liquid nitrogen. My question is how do I thaw it to keep its activity? I saw a protocol which said thaw the enzyme on ice. But some others said that the enzyme should be thawed under hot water just as thawing a cell line. So which method is better? Thanks!
Yes the enzyme can be thawed similar to cell lines, but the best way is to thaw under an immersion circulator or to keep the water in motion and at a constant temperature (water bath).
All the best
Thaw the enzyme according to the manufacturer's instructions. We prepare it as described, aliquot and keep at -20 C. We keep them for very long time until finished and always get the same yield of pancreatic islets.
Thanks
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