I am trying to understand the concept of linear dynamic range in quantitative proteomic. As I am a beginner in Proteomics.
How the dynamic range is related with the fluorescent dye for 2DE?
The linear dynamic range is the range over which ion signal is linear with analyte concentration. Ideally we would like to have a particular instrument that under a particular set of conditions is capable to measure very small as well as very high concentrations of the analyte of interest in a linear way. A different sort of dependency would make more difficult the interpretation of the amount of analyte present in the sample.
Difference gel electrophoresis (DIGE) uses fluorescence-based labeling of the proteins prior to separation. Compared to classical 2-DE (based on post-electrophoretic dye staining) it has increased the precision of quantification as well as the sensitivity in the protein detection. The sensitivity improvement is due to the fact the the new technique works with photons and there are well established techniques to count even individual photons.
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