Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View

Picrosirius red staining without picric acid?

I would like to perform picrosirius red staining but we cannot use picric acid anymore. Do anyone have already test other acid like citric acid instead? Thanks a lot

08 September 2020 2,794 2 View

Dopamine transporter antibody?

I am looking for an antibody against dopamine transporter suitable for WB. I have tried a couple of antibodies but I obtained poor results. Can anyone help me?

18 May 2020 1,536 2 View

Experience with siRNA transfection of neural stem cells?

Does anyone have experience in gene silencing through siRNA transfection specifically in neural stem cells (ReN cells)? I know these are hard to transfect cells, and with other methods I normally...

29 March 2020 4,210 0 View

Can anybody help me with Boyden chamber invasion assay for HCT-116 cell lines?

I have a problems with Boyden chamber assay for HCT-116 cell lines, I did not see any stained cell in the membrane. I have had a success with other cell line, A549. Does anyone have any...

23 March 2020 7,119 5 View

How to make cell distribute evenly in 12-well plate?

I worked with cell culture 1 year ago, nearly I have a problem when I seeding in 12-well plate, more cells settles in the middle of well. I am trying with different 'shaking method'. I hope to get...

19 March 2020 9,999 3 View

Can anyone provide the Scoring algorithm for the UK SF-12 Version 1?

24 February 2020 1,653 2 View

Can anyone recommend any strong papers concerning the prevalence of PTSD and/or CPTSD in paramedics?

Dear all, I am in the early stages of undertaking research surrounding PTSD/CPTSD and the prevalence of these conditions in paramedics. Can anyone point me towards some recent research in this...

29 October 2019 8,117 1 View

What is the best way to isolate the full leukocyte fraction from mouse blood?

I would like both monocytes, lymfocytes and granulocytes to eventually stain and stick on a slide. I used lymphoprep before, but this only gives me the mononuclear cells. I would like the complete...

08 October 2019 1,717 3 View

Could someone guide me towards my hesitation to choose my tax law thesis topic?

Good afternoon from Peru! I'm currently working on my thesis (Tax Law field), but I can't seem to decide which topic I would like to explore, because I am really interested in this three...

20 June 2019 4,419 4 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Why can't make aluminum nitride film successfully by use sputter?

We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...

03 March 2021 7,975 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View