I have tried PBS-EDTA-DTT chelation based method for isolating intestinal crypts. However, I did not achieve high yield of crypts. Can any one help me to figure out this problem?
What are you using the crypts for? if its analysis after organ culture then micro-disect them if its cell culture much more difficult
I wish to a stain a HA-Tag protein by IHC from cell-lines implanted subcutaneously in mice. The protein is very unstable by nature and expressed at low levels. Is there any good well-tested...
25 October 2020 6,007 3 View
Does anyone has prepared RNC(Rany Nickel catalyst) of 85-90% Purity? We using leaching method with NiAl alloy treated with Sodium Bicarbonate and it gives only 15% purity. What promoters,dopping...
07 June 2018 2,338 2 View
I have been working on differential expression of miRNA by qRT-PCR. I wanted to quantitate the amount of pri-miRNAs and their mature miRNAs in my cell lines before and after treatment. Is there...
30 January 2017 9,929 3 View
If highly sensitive troponin T is more sensitive, why is troponin I still used? Any references?
17 June 2013 9,132 7 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View
28 February 2021 2,534 2 View
Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...
23 February 2021 7,701 2 View
Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...
23 February 2021 9,159 3 View
I am aiming to utilise XRPD to quantify a drug excipient within a drug product and have been having a little trouble building the calibration curve to allow for this quantification up until this...
10 February 2021 6,145 3 View
Hi, I am preparing a new project on lncRNAs in human cancer cell lines. I scanned through many high-impacted journals to find the best RNA isolation kit that would also isolate small RNAs and that...
09 February 2021 4,180 5 View
I would like to isolate myenteric plexus from the rats small intestine and then isolate certains components of ENS (enteric nervous system) by biochemical tests.
04 February 2021 6,068 2 View
Hi everyone, During small RNA library preparation, I run the samples in 4% agerose gel to select the band corresponding to my target. I established beforehand the protocol and it works fine....
02 February 2021 5,008 2 View
Am working on a DMH induced colon cancer in mice and i would like to know, could polyps or ACFs be counted through visual macroscopic examination without methylene blue staining ? Thank you.
01 February 2021 4,237 2 View
Hello, In my sandwich-type ELISA which is testing for small molecule inhibition of a protein-protein interaction, it seems that DMSO percentage and dilution protocol are important. I...
31 January 2021 6,771 1 View