How do you increased the yield of isolated crypts from small intestine and colon?

07 September 2014 1 534 Report

I have tried PBS-EDTA-DTT chelation based method for isolating intestinal crypts. However, I did not achieve high yield of crypts. Can any one help me to figure out this problem?

Michael Thomas

What are you using the crypts for? if its analysis after organ culture then micro-disect them if its cell culture much more difficult

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