Any recommendations for an anti-HA tag antibody for highly unstable tagged protein IHC in mouse epithelial tissues?

I wish to a stain a HA-Tag protein by IHC from cell-lines implanted subcutaneously in mice. The protein is very unstable by nature and expressed at low levels. Is there any good well-tested...

25 October 2020 6,007 3 View

Does anyone has prepared RNC(Raney Nickel catalyst) of 85-90% Purity? We using leaching method with NiAl alloy treated with Sodium Bicarbonate?

Does anyone has prepared RNC(Rany Nickel catalyst) of 85-90% Purity? We using leaching method with NiAl alloy treated with Sodium Bicarbonate and it gives only 15% purity. What promoters,dopping...

07 June 2018 2,338 2 View

What is the best technique to differentiate pri-miRNA from mature miRNA??

I have been working on differential expression of miRNA by qRT-PCR. I wanted to quantitate the amount of pri-miRNAs and their mature miRNAs in my cell lines before and after treatment. Is there...

30 January 2017 9,929 3 View

What is the difference in clinical utility of troponin T (or highly sensitive troponin T) and troponin I?

If highly sensitive troponin T is more sensitive, why is troponin I still used? Any references?

17 June 2013 9,132 7 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

XRPD Quantification - Building a calibration curve of drug excipient?

I am aiming to utilise XRPD to quantify a drug excipient within a drug product and have been having a little trouble building the calibration curve to allow for this quantification up until this...

10 February 2021 6,145 3 View

The best RNA isolation kit for lncRNA RNA-Seq?

Hi, I am preparing a new project on lncRNAs in human cancer cell lines. I scanned through many high-impacted journals to find the best RNA isolation kit that would also isolate small RNAs and that...

09 February 2021 4,180 5 View

Does anyone know how to isolation of high-purity myenteric plexus from adult rat gastrointestinal tract for further biochemical investigation?

I would like to isolate myenteric plexus from the rats small intestine and then isolate certains components of ENS (enteric nervous system) by biochemical tests.

04 February 2021 6,068 2 View

What could be the possible causes of DNA degradation after extraction from 4% gel?

Hi everyone, During small RNA library preparation, I run the samples in 4% agerose gel to select the band corresponding to my target. I established beforehand the protocol and it works fine....

02 February 2021 5,008 2 View

Is methylene blue staining necessary for Macroscopic evaluation of the incidence of polyps in a DMH induced colon cancer in mice?

Am working on a DMH induced colon cancer in mice and i would like to know, could polyps or ACFs be counted through visual macroscopic examination without methylene blue staining ? Thank you.

01 February 2021 4,237 2 View

How does DMSO percentage affect ELISA?

Hello, In my sandwich-type ELISA which is testing for small molecule inhibition of a protein-protein interaction, it seems that DMSO percentage and dilution protocol are important. I...

31 January 2021 6,771 1 View