First of all: pituitary gland must be included in the histological section. Unfortunately you do not say anything about the "system" you (should) look into: animal, human ? Pituitary gland "per se" = real, or recognition as a tumor tissue with primary ' pituitary'?

e.g. cf: https://radiopaedia.org/articles/pituitary-metastases-1 ;

Article Pituitary metastasis as a presenting manifestation of silent...

Article Tumors Metastatic to the Pituitary Gland: Case Report and Li...

Article Pituitary Gland Metastases

If you meant "normal, regular" pituitary gland you should be aware first of handbooks brain histology etc....(practical slide course in Histology / morphology of brain?) best wishes and good luck !

Hello,

in our medical histology course we use a PAS-G-orange staining in pituitary glands from pigs to differentiate the acidophils, basophils and chromophobe cells for the anterior pituitary gland. In contrast the Neurohypophysis does not look spectacular in this histology slides.

Moreover you should also tell us about the used species (if possible).

As mentioned in the former answers, the pituitary must be included in the slide and then experience, experience and again experience.

Otherwise, if you are the person who performed the preparation of the organ, you should be easily able to dissect it from the skull and then you know its the right organ.

If you have a specific histology-slide and you would like to know if it is the pituitary gland why don't you post an image in an overview and a higher magnification image here? Then someone could perhaps help you.

Another nice source for histology slides is: http://histology.medicine.umich.edu/full-slide-list

Sincerely Yours

Stefan

Hello,

Thanks for all the answers. I was looking into a normal pituitary gland of a human. I guess I will need more experience, for I currently have not had much practice. I found an atlas on histology and looked at the pituitary section, however the book mainly talked about the parts of the gland. I was wondering if their was a key identifier that people with experience would immediately go to. There was not a specific slide I was looking at with a specific stain, I was just trying to find a key identifier when looking at a close-up of acidophils, basophils, and chromophobes. I guess my question was a bit too vague.

Thank you!

I agree with the above answer of Stefan Jean Pierre Hass. I have some experience of sectioning and staining of amphibian pituitary. Pituitary gland of vertebrates is sectioned in sagittal plane and stained using Alcian Blue-Periodic acid Schiff's-Orange G counter to differentiate and identify cell types, basophil s, acidophils and chromaffin cells of adenohypophysis. In a stained section Pars Distalis looks very distinct from neurohypophsis and surrounding brain tissue