when one gets CD34 cultured in methocult, one can pick the different colonies like E-BFUs and E-CFUs or GM-CFUs etc. and stain the cells with Giemsa/may Grünwald etc. The question is what would be the best protocol? do you direct put the colony on the slide and fix them there, or does one need really to wash them and prepare the cells as cytospins? the cytospin method would be better to evalute the cell morphology, however we are interested in the colony morphology, so how the cells are growing there, and this would be completely destroyed after wash. so I was wondering whether one can directly stain the picked colonies. many thanks! Oliver Schmetzer
The best way is to pick similar colonies such as BFU-e colines and wash them and then do cytospin. It will help you to see the cell morphology in individual colony as well different type of cells in the same colony.
- Badges
- Science topic
- Similar topics
- Biological Science
- Immunology