Zahir Shah

4 Questions 37 Answers 0 Followers

Questions related from Zahir Shah

How to improve the expression of GST tagged protein?

We have constructed GST-tagged protein to the N-terminal of GST in pGEX-4T2 plasmid which is about 70-90 KD. We tried different concentration of IPTG for induction but the expression is not...

06 June 2014 2,169 2 View

How can I overcome the high uniform background in western blot?

I am getting a high background problem with western blot. I have given long time for blocking and washing with 1% TBST thrice for 10 minutes. But the result does not improve and the background is...

03 March 2014 1,514 7 View

Less Opa1 isoform bands after Western Blotting?

I have performed Western Blotting many times for getting the five bands for opa1 isoforms in HeLa and HeLa rho zero cells by running the samples on 12,15 and 18% gel for 8,10,12,14,16 and 18 hrs....

12 December 2013 2,032 2 View

Can anyone help with protein-protein interaction?

I am doing CoIP to determine the interaction of my protein of interest (Flag tagged) with another protein. I used the primary Ab against Flag and after WB, the result was okay but at the same time...

12 December 2013 6,824 4 View