I used capillary tubes to do thermal death time test, and it is very difficult to get the sample (40uL) out of the tube. I tried pipette it but still small amount of liquid inside. Any ideas to get the sample out the tube in a more complete way?
Is it by suction or blowing? Usually, people shake them hardly! It has to have enough time for drying!
I usually do that by heating the sample and applying high vacuum. To do that you can utilize a vacuum camera and put off your sample inside.
Jie, I am aware of 2 options but not sure how convenient it is for your samples. 1. cut the sealed side of the tube to release the sample, you can retrieve it with a pipette. 2. place the tube in a falcon tube (or Eppendorf tube if your sample is small enough) and do a spin in a centrifuge it should take your sample into the larger tube and you can move from there DO THIS AT LOW RPM for safety.
You can prepare a little bit more sample, says, 41 uL, in the capillary. Trying to get the sample taking out closer to 40 uL.
There are capillary plungers you can buy...inexpensive and disposable.
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