Can anyone suggest a procedure for determining the total phenols content?
Article Antioxidant Capacity and Phenolic Content of the Aqueous Ext...
Hy Bhuvana,
fully described method originally proposed by Amerine and Ough (1980) for determination of total phenols could be found in my paper :
https://www.researchgate.net/publication/221842380_Seasonal_variations_of_phenolic_compounds_and_biological_properties_in_sage_%28Salvia_officinalis_L.%29
For the determination of extract concentration you need to evaporate the solvent (by vacuum evaporator or lyophilisator) and than dissolve the crude extract in the known volume. Usually the concentration used is 1 mg/mL so it is easier to compare the result with those published by other authors.
Article Seasonal Variations of Phenolic Compounds and Biological Pro...
I agree with Mr. Rana. For determination of phenolics, this is mostly the first step which is followed by most researchers. The FC method or the Gallic Acid Equivalence method (GAE), is a mixture of phosphomolybdate and phosphotungstate used for the colorimetric in vitro assay of phenolic and polyphenolic antioxidants.The reagent does not only measure phenols, and will react with any reducing substance. It therefore measures the total reducing capacity of a sample, not just phenolic compounds. This reagent is part of the Lowry protein assay, and will also react with some nitrogen-containing compounds such as hydroxylamine and guanidine.
After solvent extraction evaporate the solvent on a vacuum evaporator or lyophilisator. Dissolve the crude extract/precipitate or powdered material in the known volume of solvent. . The total phenolic content (TPC) are estimated spectrophotometrically using Folin Ciocalteu method. Total flavonoid content (TFC) is
measured by aluminium chloride colorimetric assay.
After evaporation using rotary evaporator the minimized amount of extract is dried using lyophilizer. The dried powdered form of extract is then can be used to prepare stock concentration as and when needed.
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