I wanted to measure the quantum yield of singlet oxygen by phosphorescence. In this regard my questions are:
1. How much concentration is required?
2. Do I need to measure both in aerated and non-aerated solvents?
Dear Sabarinathan,
for the 2nd question please take a look here http://www.nist.gov/data/PDFfiles/jpcrd449.pdf
for the 1st I am using other methods for singlet oxygen yield determination but for sure you should avoid aggregated form of the dye in your solution. What kind of photosensitizer you plan to use?
I hope it will help you
Hi Michal,
thanks for the link. I am working on porphyrins for PDT application.
Hi Sabarinthan,
Please see my papers about singlet yield determination , by chemical and physical methods.“Water-soluble non-aggregating zinc phthalocyanine and in vitro study for photodynamic therapy”.
ChemComm.49 (2013) 11149-11151.
“Tetra- and Octa-substituted Phthalocyanines Possessing Bulky Phenoxy Substituents: A Comparative Study among their Photophysicochemical Properties and Aggregation Behavior.”
J. Porphyrins and Phthalocyanines. 16 (2012) 163–174
For second you need to measure them in non-aerated solvents.
Dear Ahmad Tuhl.
Can you please describe more precisely, how to determine a yield of singlet oxygen generation in non-aerated solvents?
Dear Sabarinathan,
Yield of singlet oxygen generation is usually (in not saturated conditions) only slightly dependent on the concentration of photosensitizer, because it is just a coefficient. But the total amount of generated singlet oxygen will directly related to concentration and irradiation power.
Do you need to determine the amount (for a practical usage), or to determine just a photophysical characteristics (theory, dye characterisation, etc.) ?
Dear Sabarinathan,
It is not really clear, phosphorescence of which species you are going to detect. Do you mean phosphorescence of a sensitizer, or the phosphorescence of singlet oxygen?
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