How do you design a primer for an RNA target?

16 April 2014 1 7K Report

We are trying to develop a biosensor to detect the Dengue virus (DENV) in field condition. Since DENV is an RNA virus, I need to design primers that can specifically bind to RNA. These primers are not for the purpose of any form of PCR but only to capture DENV.

Sneha Singh

Hi Shishir.............. I believe you can use the same primers as we use for RT PCR to detect RNA in the field since DENV RNA lacks a poly A tail, we use its specific reverse primer for preparing the cDNA. You can take help from Anoop M et al., 2010; 2012.

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