Hello there, I would like to correlate the mRNA expression of a gene obtained from patient blood samples with surrogate parameters like HbA1c and data like body weight, age, etc. However, I am unsure and confused about the correct way to do it. I did not find a solution on the web so far.
Which qPCR-data should you I use (dCT, ddCT, 2^-ddCT)?
If it favorable to use log-transformed data, or does it distort the results?
Is it statistically correct to simply use an xy-graph and do a correlation analysis?
Regards!
I can tell you that qPCR data can be correlated with patient characteristics by using statistical analysis. You can use correlation analysis to determine the relationship between two variables. In this case, you can use correlation analysis to determine the relationship between qPCR data and patient characteristics.
To correctly correlate qPCR data with patient characteristics, you can follow these steps:
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