I have bio-synthesized a bialloy metal manoparticle and now I want to cap this nanoparticle with a drug .
There are some method which I learnt but all were for the chemically synthesized nanoparticles.
it depends on what your drug is and what your biomolecules are that you have used for the biomineralisation of your particles. EDC/NHS can be used to couple carboxylic acids in your biomolecule to an n-terminal amine in a protein drug, or a lysine added to a synthetic drug. You could also biotinylate your biotemplated particles using the same strategy. There's loads of options.
The FTIR analysis of the metal nanoparticle showed the presence of OH stretching, C-H stretch, N-H bend and C-N stretch, that are playing role in the capping of these metal nanoparticles. I want to combine streptomycin to it. Streptomycin also has OH, NH, strech.
Is it possible to couple both with the EDC/NHS
the streptomycin would have to have an amine on it e.g. Be functionalised with a lysine, for edc nhs to couple it to any carboxylic acid in the coating. It is not the parts of the biomolecule that are bound to the particle that you will couple to, but to the ones away from the surface that are free for binding to the external molecule. Ftir won't help tell you what these groups are. Is your biotemplate a protein? If so, it will contain carboxyllic acid. If it is lipid, you'll need to find out what the lipids are to determine what chemistry might bind to it. If there are cysteines, their sulfur might also be useful. You need to know what your biotemplate is to find out what you can do to bind things to it. Also, binding can reduce or stop a molecule from working, so you might have to add spacers or linkers to make sure it still has functionality.
I am using cell extract, which contains different kind of proteins and enzymes (not characterized) for the biosynthesis of the metal NPs
It sounds like you might just have to try the coupling and see if it works. Good luck.
You have used bio-extract (plants or bacteria etc.) and metal precursors during synthesis process. After completion of synthesis, before separation of metal nano-particles from the liquid, add the Streptomycin. Proteins have strong affinity towards metals. For details:
https://www.researchgate.net/publication/233981900_Konjac_Bio-Molecules_Assisted_Rod-Spherical_shaped_Lead_Nano_PowderSynthesized_by_Electrolytic_Process_and_Its_Characterization_Studies?ev=prf_pub
Article Konjac Bio-Molecules Assisted, Rod-Spherical shaped Lead Nan...
Streptomycin guts guanidine groups an as we know amine and guanidine groups interact with metallic nanoparticles. It seams, you can couple you particles with streptomycin simply by mixing them.
For example you can find interaction of antibiotics with surface of silver nanoparticles in the literature.
Generally water soluble drugs can be attached with colloidal green synthesized nanoparticles by simple mising as proteins from plant extract act as coating agent. Although this kind of binding is not strong and % attachment of drug also less, but as you are using green synthesized nanoparticles you can use more concentration of it for therapeutic doses.
See my paper:
Green chemistry approach for the synthesis and stabilization of biocompatible gold nanoparticles and their potential applications in cancer therapy
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