Hi,
I need to determine that the cells I have isolated are adipocytes. Has anyone done this or know how to do it by immunocytochemistry?
Many thanks,
Mel
Usually is easy to know that it an adipocyte by your celular form but you can do a Oil Red tincion or detect by AP2 marker.
Thank you Veronica
Try this http://www.abcam.com/adipose-triglyceride-lipase-antibody-ab85858.html
Thanks Dmitry!
After COVID-19 it has seen that EFL learners technological affiliation has raised. In addition, in the post-COVID period learners started to engage AI technologies like ChatGPT while learning...
08 August 2024 8,964 4 View
How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...
08 August 2024 6,690 3 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I want to refine one XRD peak of my in-situ xrd but the background is never working good which ultimately fails the refinement. How to refine and adjust the background using GSAS-II
05 August 2024 5,291 2 View
Hi, i would like to simulate an absorption process in Aspen Plus. I want to use the NRTL model und would like to add some individual Henry coefficients. Is that possible and how?
05 August 2024 2,333 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
AI tools like ChatGPT can enhance research work significantly when used responsibly and in conjunction with thorough human oversight.
05 August 2024 1,842 3 View
Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...
05 August 2024 3,084 6 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
Citi BLOC Standard Basket Definitions: A standardized unit representing a fixed basket of construction materials, labor, and equipment costs priced in various cities. Purpose: To create a common...
04 August 2024 8,997 1 View
I have multi-stage data. The ICC is different at all level. Say at stage 1 =0.1, at stage=0.2 and at stage 3=0.3. I want to run melogit regression. Please, which variable should be in...
16 June 2024 6,265 4 View
Hi there! We are trying to use the Concentric Circles Plugin on ImageJ to determine signal intensity in neurosphere cryosections after immunocytochemistry/confocal analysis. We determined the...
12 June 2024 7,332 0 View
I am using the hanging drop method to develop individual differentiated adipocyte spheroids from the stromal vascular fraction of adipose tissue. With our current timeline they end up 100-300um....
27 May 2024 1,303 1 View
hi have you ever experienced of coming different results from western blot and icc? I treated toxin (a-syn PFF) to control ES-derived midbrain doparminergic neuron and experimental group that I...
25 May 2024 3,162 0 View
Hi, I have been working with 3T3-L1 cells for over a year now.However, over the last couple of months they stopped differentiating into adipocytes. I seed at 200k cells/well (12 well plate), and...
23 May 2024 3,356 1 View
I have an antibody specific just for ICC and WB but I should use the same antibody to perform an IHC staining. Is it possible to use this ab also in IHC?
15 May 2024 6,392 3 View
I am evaluating histological slides (H&E) of brown adipose tissue from rats. And counting the number of unilocular adipocytes to evaluate the whitening process. How to identify? Would it...
13 May 2024 7,876 0 View
As human adipocytes can be up to 300 μm in diameter, How these cells can be analyzed for cell markers by flow cytometry with 100 micron Nozzles? What changes are necessary to be done on flow...
12 May 2024 2,254 0 View
Dear Researchers What are the initial characterisations utilised to confirm the synthesis of MOF. Kindly suggest. Thank You
24 April 2024 1,425 2 View
I have collected data at community level using cluster sampling. The ICC shows >10% variability at cluster level. However, I don't have relevant variable at cluster level (all variables are at...
22 April 2024 8,005 2 View