How do I separate cells grown on collagen for western blot and also how do I know if cells are confluent if grown on a translucent membrane?

Working on Caco 2 cells. I want to know about the cells grown on collagen for differentiation. Also, can I perform a lucifer yellow assay for assessing the permeability followed by western blot in...

02 March 2018 4,615 5 View

Why do my Balb 3T3 cells die after I try to subculture them from the transport plate into a new flask?

I am planning to work on 3T3 cell line. I acquired them in healthy condition but started dying with structural changes when I tried to sub culture it. Serum was complement inactivated and all the...

05 June 2014 6,200 14 View

Can anyone suggest if there are any FACS kits to count the T (sub sets) and B lymphocytes, NK cells in rat blood and spleen cell suspension?

I am planning to carry out immunotoxicity studies in pesticides and as a part of the study i want to measure the subset population and their variations, live and dead count using FACS. Is there...

05 June 2014 1,238 3 View

Can anyone please suggest me some new compounds (medical or agriculture related) of interest which needed reproductive and developmental toxicity?

I am planning to work on toxicity in rats. can anyone suggest me some compounds in development process preferably in agriculture related such as insecticides or pesticides, etc.

02 March 2013 2,304 1 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View