Hello. I have been working on a study that is exposing N27 and SH-SY5Y cells to alpha-cypermethrin, a combination of two isomers. It seems to generate two peaks on achiral columns. We have attempted to get a single peak, but it does not appear to be possible with our setup. One isomer peak area is very small in my standard (ratio of isomers 10:1). After running 24 hour cell exposure experiments the ratio of the isomers equilibrate to a ratio of 1:1. This has commonly been seen in the literature for pyrethroids when they are in mixtures of water and a solvent. When I go to calculate my recoveries, if each peak is treated as a single compound the isomer that has the peak area that increases is greatly overestimated and the other one seems to be underestimated. This is because my reference standard is stable at the 10:1 ratio. What I have been doing is taking the sum of the peaks and treating it like a single compound, but I do not know whether this would be a correct way to calculate the recoveries. Is there an accepted way to integrate peaks when isomeric conversion occurs? Thank you for any ideas!
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