Does CD31 indicate cell viability?

Hi, I am wondering if the immunofluorescent staining of CD31 antibodies can both mark endothelial cells and indicating their viability. In other words, if I perform immunofluorescent staining for...

23 December 2020 5,815 4 View

Analysis and implementation of Kalman filter in fixed-point arithmetic?

I would like to implement a Kalman filter for tracking applications for IoT nodes. I cannot do floating point on our nodes but only fixed point. Could you recommend some good references?

23 December 2020 4,813 3 View

ELISA detection cytokine in human serum (common dilution fold)?

Hi everyone, I'm gonna try to detect the IL-10, IFN-y, TGF-beta1 level in the human serum by ELISA. I bought the ELISA set from the BD contain Capture Ab, Detection Ab, Enzyme reagent, Standards....

09 December 2020 3,369 5 View

What is a popular and standard model for quantifying land change drivers/determinants?

I'm looking for a standard model to quantify the land change drivers (e.g., forest to agriculture or vs). Actually, it depends on the data that I have and the specific location, but I would like...

22 November 2020 7,736 3 View

Which book should be best as a text book for Health Psychology in university?

Hi everyone. I have read some recommendations about the book of Health Psychology for teaching this topic at university. These were posted in 2013, thus, I ask this question again if there is any...

07 October 2020 1,290 7 View

The correctness of synthetic data and corresponding label generating from smote or adasyn ?

Dataset imbalance is a major problem usually encounter in machine learning practice. Some technique such as SMOTE or ADASYN generate addition data (synthetic data) to avoid imbalance problem....

06 September 2020 3,337 3 View

How to convert a SAED pattern into a 2D plot like XRD profiles (using GMS3)?

I try to use the line profile, but it just intersects with a few spots in the whole SAED pattern. Is there any way to sum all the spot intensity as a function of distance in reciprocal space?

12 July 2020 7,178 2 View

Multinomial (Logistic) Regression in SEM in R?

I was wondering there is a way to carry out multinomial logistic regression within a structural equation model in R? I am currently using the lavaan package, and so far have not been able to find...

28 June 2020 4,813 3 View

How to modelling a Mass-spring-damper system of two-body Point absorbed Wave energy converter using Ansys Aqua?

Dear Colleague, I would like to simulate a two-body FPA wave energy converter using Ansys Aqwa. Unfortunately, I have no idea to simulate a mass-spring-damper system that represents a PTO system...

05 June 2020 8,959 1 View

Put nanoparticles into nano Hole?

I have a metal (Silver) Hole Array (thickness 200nm) with the Hole radius about 200nm. Also, I have non-magnetic (Pd) nanoparticles dissolved in the solvent (hexane). Is there any way to put the...

27 April 2020 6,043 8 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How two melting peaks in DSC of polypropylene can be merged into one crystallization peak?

The figure shows DSC cooling (blue) and 2nd heating (green) curves (step: 10 K/min) for a commercial sample supplied as Atactic Polypropylene (APP). The heating curve illustrates two major...

03 March 2021 5,857 3 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

What is recommended for solid domain in ANSYS FLUENT?

Should I mesh the solid domain ? or should I use wall thickness? or Shell conduction? for Heat Transfer between two fluids of a Heat Exchanger. what is recommended?

01 March 2021 9,785 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How can I make stacked bar graph with scatter plot?

I want to make a stacked bar graph with showing each data value as dots. I tried with graph pad prism, version 6, but could not succeed. Does anyone have tried before making such graphs?

01 March 2021 9,383 1 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

How can i resolve the "final solutuin is not converged" error in Comsol for my heat transfer simulation??

I have been trying to simulate heat transfer problem in Comsol 5.5 for long time but it always throw an error stating: "Failed to find a solution. Maximum number of Newton iterations...

28 February 2021 8,626 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View