How do prepare APGal synthetic minimal media for yeast??

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If my gene of interest has high GC content can it be problematic in sequencing? What kind of error is expected with GC rich gene sequences??

Gene sequencing related trouble shooting

25 July 2024 4,149 2 View

Can someone provide the detailed protocol for the synthesis of lanosterol nanoparticles?

There is no information present in the databases regarding the synthesis of lanosterol nanoparticles.

28 April 2024 2,079 3 View

Is 2D-DIC sufficient for assessing damage on carbon fiber-composites in compression after impact test?

We have a 2D DIC setup and want to know if there are any open source/commercial packages which can help us assessing the out of plane deformation due to compression of the coupon (carbon fiber...

21 November 2023 2,850 0 View

How to generate random pores within a CAD geometry with a desired porosity and pore sizes ?

I have a solid CAD model geometry (complex shape). I want to generate random spherical pores within that geometry with desired porosity and pores size.

11 May 2023 6,275 2 View

Is it normal for organoids to form clump in differentiation culture?

I am trying to make midbrain organoids from iPSC. After matrigel embedding, when I transferred organoids to ULA 6-well plate for differentiation. I found organoid-formed clumps in the media. Is it...

25 April 2023 7,739 1 View

How to use references in latex with standard templates?

I am a newbie to latex. I was trying to use references in latex. I was attempting to have the author's name and the year of publication appear in my literature section in place of reference...

26 January 2023 8,360 2 View

Latent Heat of Manganese Dioxide (MnO2) ?

I am looking for the heat of the fusion of manganese dioxide. I am unable to find it in the literature.

11 January 2023 5,812 4 View

Which cruicble can be used for TG-DTA of NaOH(s) samples ?

I want to perform TG-DTA on NaOH in an inert medium. Generally, alumina and platinum crucible are used for TG-DTA. However, I am confused with respect to crucible material compatibility with NaOH...

12 December 2022 6,754 2 View

What are the mega trends and recent debates in SPIONs Contrast Agents for MRI?

SPIONs has been extensively explored as the Contrast Agent for MRI. What are the current mega trends that are in this field? What are the challenges that are still to be achieved and overcome?

05 September 2022 7,207 4 View

Most contagious and contaminat dyes for study in Photocatalytic dye degradation?

Hello RG experts I am working on dye degradation and looking for dyes which are suitable for todays scenario. Could you all please enlist some of the most contaminant dyes that are suitable for...

28 January 2022 1,331 7 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View