hello:

i have been working on hard tissue for a long time most of my expertise  is on teeth but i also worked on bone .

we have been using two machines  one is  old and is called the Gilling Hammco machine  and they still use it in my oral pathology department (see picture below)  i moved from the Gilling Hammco machine to  the isomet machine  .we usually embed the specimen in methyle meth acrylate  (MMA) clear  using a embedding wax plastic boxes (which are used for regular paraffin  sectioning we use them to fill them with epoxy and place the teeth or bone in the way we want to be sectioned .

once the MMA hardens we remove the plastic cover and now we have  a cube of MM embedded inside it the tooth or bone.

using the Isomet machine we can cut sections thickness of almost 7 microns .(i have enclosed a sample here also of  a sectioned tooth about 7 microns thick.

here is a paper using bone and Hammco machine 

explanation of image item 1,tooth,item 2 is giling and Hammco machine ,3is the isomet machine,#4 sections of teeth #5 an actual light microscopy of enamel section about 7 micron thick

wray JB: vascular regeneration in the  healing of fractures: an experimentakl study: 

angiology14-134-138,1963.

attached here is the isomet machine i have used and a section of almost 7 microns . i can provide papers i published on teeth sectioning using this machine if needed.

best wishes

Prof Galil