Unloading stifness degradation?

Hello friends, In the scope of my master thesis I am developing a numerical model for a steel connection under cyclic loading. I already calibrated the plastic and damage properties of the...

02 December 2020 2,201 2 View

I would like to ask some questions about concentrations of Urea 3M and 8M in two experiments?

I would like to ask if anyone knows if concentrations of Urea 3M and 8M can interfere with protein purification at Ni-NTA and Glutathione Sepharose 43 resins. Another question, Urea, at 3M and 8M...

17 November 2020 5,014 3 View

I would like to ask if anyone among you knows what is the ideal concentration of TFA to use at a chemical synthesis of peptides?

During a chemical synthesis of peptides by the F-moc method, the following happened: all the initial steps of the synthesis occurred as planned, but in the cleavage, TFA is used. In the lab, there...

02 November 2020 2,997 8 View

Is there any software to simulate viral infection of cells?

Hello, I am looking for a computational simulator of in vitro infection of cells by virus, to be used for teaching purposes. Do you know any tools/resources ? I would like students to set...

13 April 2020 2,144 1 View

Incremental Conductance vs P&0, for Solar MPPT?

Hi I am studying IC and P&O in a boost converter for solar panels. I am startled because I get nearly identical results. Does anyone know why can't I see pratically any deviation between...

10 March 2020 1,883 4 View

Does anyone has a good experience growing the TALL-104 cell line?

We bought this cell line 6 months ago and we are trying to make it grow, no success so far. We are using ATCC suggested media and replacing IL-2 every 3 days. Fully media changes (by centrifuging...

13 January 2020 7,719 1 View

EO771 mouse tumor transfection protocol?

Hello! I've been trying to transfect EO771 using lipofectamine 2000, but despite changing the transfection media after 6 hours of incubation I have a high rate of cell death. My untransfected...

13 November 2019 7,883 3 View

Lift and Drag Coefficient too low on airfoil simulation - ANSYS Fluent - Where is the problem?

I have been modeling some airfoils to test its aerodynamic properties and when comparing to air tools database the values are very different. I was expecting the lift coefficient somewhere near...

22 October 2019 6,106 3 View

How can I calculate Ethanol's boiling point for a specific volume?

08 October 2019 7,724 3 View

How can avoid aggregation of cells in E. Coli staining with ThS and propidium iodide when trying to visualize them in an epifluorescence microscope?

I got competent cells of E. Coli induced with IPTG to express B-amyloid peptide and I stained them with ThS and propidium iodide after permeabilizated with Triton x 100; however, when I want to...

07 August 2019 3,158 1 View

Do you need permission to use a middle range theory in your dissertation?

I am using a figure of a middle range nursing theory, do I need permission to use?

03 February 2021 2,210 9 View

What will be the impacts of post covid-19 stress on working nurses?

The health and well-being of nurses are essential to the quality of care that they provide for people and communities. The Covid-19 pandemic has put them under unprecedented pressure. The impact...

26 January 2021 2,440 20 View

Hello, my name is siti. My question is what is the related issue in bone marrow transplant or hematology that I can choose for my research project ?

I am doing a master in nursing but still does not have any idea about what topic I am going to choose. pls help

25 January 2021 1,097 12 View

(ORGANISATIONAL FACTORS ) request for study instrument?

i am currently conducting a study to assess bullying behaviors on student nurses during clinical placement . My objective is to explore organisational factors that influence bullying behaviours at...

25 January 2021 8,676 1 View

How to conduct Methodological Research?

Hi everyone, I plan to create a design for a system using previously published articles, journals, etc as a basis. I found methodological research design but I don't know how to conduct it. Like...

07 January 2021 5,824 3 View

How Healthcare Personnel help frontline workers cope with stress and mental burden during COVID-19?

Which methods does they suggest to ease the burden and stress as a teamwork?

09 December 2020 9,605 7 View

Special Issue: New HRM Models for Supporting Managing Emotional Labour During Emergencies?

IJHRM has called for a special issue on the subjected-topic. If anyone is interested in working on this special issue, please contact me. Scholars should have a grip on comparative analysis and...

09 December 2020 8,784 4 View

How to approach native speaker to be a co-author and how to build trust?

For non-natives, a manuscript is often rejected based on the reason that idea is not presented clearly. Please ask a native colleague to proofread it. There is no offense to say that I am...

26 November 2020 7,495 12 View

Self-Efficacy VS Emotional Intelligence?

I am working on my dissertation topic with the below sections: - Anxiety Buffer Hypothesis - Self-Efficasy - Emotional Intelligence - Covid-19 Anxiety or Fear Has research investigated...

20 October 2020 7,823 2 View

Self-Efficacy VS Emotional Intelligence?

I am working on my dissertation topic with the below sections: - Anxiety Buffer Hypothesis - Self-Efficasy - Emotional Intelligence - Covid-19 Anxiety or Fear Has research investigated...

20 October 2020 643 4 View