Working on SCCmec typing of MRSA isolates. When I carry out multiplex PCR for this purpose, some of the isolates' gel electrophoresis results contain only one band, which belonging to mecA gene (162 bp). Can this isolates be grouped under a SCCmec type?
My primers are from 'Milheiricio et al, Update to the Multiplex PCR Strategy for Assignment of mec Element Types in Staphylococcus aureus', and I use mainly this study.
The scheme of Milheirico et al is fairly good for fine subtyping the SCCmec. However, in our lab, we use a simple approach to differentiate between the major types I-V using the primers described by Boye et al. I attached the link to the article. Hope it will work
http://onlinelibrary.wiley.com/doi/10.1111/j.1469-0691.2007.01720.x/full
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