Hi Jada, it will be difficult to isolate a  protein from RBCs without Hb since Hb is ~95% of the total RBC cell protein.

Since Hb has a MW of ~65kDa, can you use size exclusion filters to isolate your protein?

Hi Jada,

Please specify your aim more precisely.

You want to clean the cell membranes  or intracellular proteins from hemoglobin?

@Gregory I would want to aim towards cleaning cell membrane from hemoglobin. @Steingrimur I am not sure. Well if I can not exclude HB, do either of you know a good protocol for just  isolating protein from RBCs?

Hi Jada, you said that you were going to "quantify a particular protein in red blood cells".

Depending on what protein you want to quantify, there might be ELISA kits out there that have been developed to quantify your RBC protein in lysed cells. 

It would help if you can divulge what protein you are going for.

p-ERK 1/2

Hi Jada, here are some companies that sell p-erk 1/2 ELISA kits.

I hope this helps!

http://www.ebioscience.com/catalogsearch/result/?q=erk&order=name_sort&dir=asc&gclid=CMHBv8L5yMMCFQwjgQodZwkAeA

http://www.perkinelmer.com/catalog/product/id/tgres10k

http://www.blossombio.com/pdf/products/UG_AlphaScreenSureFireERK12pThr202Tyr204AssayKit.pdf

http://www.abcam.com/ps/products/119/ab119674/documents/ab119674%20PhosphoTracer%20ERK1%202%20pT202%20Y204%20p38%20MAPK%20pT180%20Y182%20JNK1%202%203%20pT183%20Y185%20ELISA%20Kit%20(Website).pdf

Thank you

On both soluble and membrane protéine extracts from RBCs, I successfully depleted Hb through IMAC chromatography with nickel-loaded phase, the same way as for the purification of His-tag proteins. Have a look at this paper : 

Journal of Proteomics

5 December 2012, Vol.76:181–193, doi:10.1016/j.jprot.2012.05.004

hope that can help you ;-)

@Julien. Thank you