Jada Selma

4 Questions 7 Answers 0 Followers

Questions related from Jada Selma

How do I separate out all other proteins from hemoglobin in red blood cels ?

I am attempting to quantify a particular protein in red blood cells and I want to remove hemoglobin so that it will not interfere with the assay that I am using.

02 February 2015 2,800 9 View

Can you purchase specific phosphatases like you can inhibitors?

I am trying to inhibit the phosphorylation of a Erk 1/2 but the cells I am working with naturally have it already phosphorylated. I want to see if I can remove the phosphate group with a specific...

12 December 2014 5,054 3 View

Is it possible to put a fluorescent tag on a protein in order to track if its transferred to neighboring cells ?

I am trying to label a phosphorylated protein in one cell type and then image if the protein is transferred to another cell type after co-culture.

11 November 2014 6,670 3 View

Is there another stain besides Filipin III for cholesterol?

I am trying to stain cholesterol in THP-1 cells, but I have heard the Fillipin III photobleaches very quickly and it is hard to see in under confocal

11 November 2014 2,814 2 View