What method can we use to remove chlorophyll without degrading the antioxidants in it?
To the best of my knowledge, it is impossible to remove chlorophyll without damaging antioxidant enzymes. You have to use 80% acetone or ethanol or DMSO for removing chlorophylls and all these organic solvents will damage enzymes.
There is what is called non-destructive method of chlorophyll determination. Please follow that. Still I believe antioxidants will be removed. Chloroplast itself contains lot of antioxidants. When chlorophyll is removed from membranes, automatically antioxidants will be leached out
puede utilizar clorolactofenol d´AMANN , en las proporciones siguientes: 2 de hiodrato de chloral 1 de phenol y 1 de ácido láctico
Use 80% acetone or DMSO. What is your aim of removing chlorophyll from the leaf?
i just want the antioxidant in that leaves for my future research,mr. karuppiah and the chlorophyll pigment will effect my result if i not eliminate it.
Many followers of the question have answered well. The chlorophyll and carotenoids can easily be removed from Pandanus by either 80% acetone! DMSO or even EtOH .
I think we are drifting away from main objective (i.e. removing chlorophyll from Pandanus amaryllifolius leaves without degrading antioxidants). Using organic solvents (like acetone, ethanol, methanol or DMSO) and clearing agents will damage enzymatic antioxidants. Therefore, if any one has an alternate method to remove chlorophyll from these leaves, kindly suggest. Photobleaching can lead alteration (in general an increase) in antioxidants (both enzymatic as well as non-enzymatic). I do not know, if etiolated seedlings can be used (i.e. seedlings raised in dark). However, etiolated seedlings generally have smaller leaves and I am not aware of antioxidant status of etiolated seedlings (I am sure darkness will influence gene expression significantly and hence expression of genes that are needed for production/synthesis of both enzymatic and non-enzymatic antioxidants).
Chlorophyll can be extracted with acetone. Working in a dark fume cupboard with very limited or n light will reduce any enzymatic breakdown.
- Badges
- Science topic
- Similar topics
- Biological Science
- Plant Sciences