I am trying to isolate a nerve growth factor, and the protocol calls for various flow rates throughout the process. I have a really old flow adapter in the column, but I really cant seem to regulate the flow to match the protocol.
Is there anything I can do? Perhaps a pump that regulates the flow?
By using pump you can regulate flow but if you are using present column then before experiment you have to do some trails and fix this this problem.
Dear Ronny,
Are you using a glass column, how is liquid passed through the column. What is your detection technique. Please provide more details.
Kind regards,
Ade Kujore
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Not enough information to answer you properly. how ever I suggest to wash (high speed) 100 cm/hr and when you have to work slowly go down to a flow of about 50-60 cm/hr. Indeed as mentioned above the best way to regulate the column flow is by a peristaltic pump.
Good Luck
Of cause a peristaltic pump with the variation of spead. But if not enogh (too high or too slow) you may change the diameter of the tubes applied in the pump. You may calibrate it by weighing the passed liquid volume collected in given times.
The ideal flow rate for an ion exchange column is 1 mL.min.cm-2. You must know the cross section of the column to calculate this flow. The control must be done by a pulse or peristaltic pump.
Hi Ronny,
It depends on the amount of sample. If you work with low volumes like me, you can easily fill a colum with your resin and adjust the effluent flow by a valve at the bottom of the column. This way you prevent flow channelling too because you have a head of water above you resin bed. Picture is attached. Hope this helps you.
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