Ronny Khalil

5 Questions 4 Answers 0 Followers

Questions related from Ronny Khalil

How to quantify bacteria using a spectrophotometer?

I am attempting to construct a standard curve to determine the concentration of bacteria in lactose broth. I concentrated the starting lactose broth, made serial dilutions, plated each dilution...

22 June 2017 7,375 4 View

How do I prepare 0.1M sodium Borate buffer, pH 8.0?

I would like to carbamylate LDL, and need to use 0.1M NaBorate pH 8.0 I would appreciate feedback

22 January 2016 9,901 10 View

How do I regulate the flow in an ion exchange column?

I am trying to isolate a nerve growth factor, and the protocol calls for various flow rates throughout the process. I have a really old flow adapter in the column, but I really cant seem to...

27 November 2015 1,831 7 View

What stain is best recommended for staining HLPDS in agarose gels?

I use a lipoprotein stain to visualize various lipoproteins on agarose gels. Given that HLPDS is void of lipoproteins, we don't see any bands in the lane. Is there a way to stain the gel to...

04 March 2015 1,049 3 View

What is/are the likely reason(s) for the separation of the fluorescent DiI and LDL?

Hello everyone, I made low density lipoprotein (LDL) labeled with 1,1'-dioctadecyl-3,3,3',3'-tetramethyl-indocarbocyanine perchlorate (DiI) and prepared for macrophage like cells (P388D) for...

18 December 2014 8,008 1 View