Hi,
You can use our freely available tool (plugin), IHC Profiler, to quantify DAB stain for markers wherein the localization is either nuclear or cytoplasmic.
https://www.researchgate.net/publication/262111731_IHC_Profiler_An_Open_Source_Plugin_for_the_Quantitative_Evaluation_and_Automated_Scoring_of_Immunohistochemistry_Images_of_Human_Tissue_Samples
Good luck.
Amir
Article IHC Profiler: An Open Source Plugin for the Quantitative Eva...
AFAIK, peroxidase reaction and DAB deposition is not stoichiometric, so it is not possible to quantify say 'amount of reaction' as a function of antigen abundance. I guess what Amirali suggests is a quantification of amount of positive cells.
Probably if you would like something more appropriate for quantitation you could use alkaline phosphatase and tetrazolium salts as it is done for in situ hybridization. There, the precipitation of the formazan is something that could be quantitated, when not saturated (which is crucial, meaning that you should stop the reaction before you loose linearity).
Dear all,
Did someone find the method used by Dr. Mustafa published or written somewhere? I would like to take a look to a more formal SOP or perhaps to a paper including this method.
Thank you
There is also a IHC toolbox plugin, https://imagej.nih.gov/ij/plugins/ihc-toolbox/index.html
This one used deeplearning to enhance the effect, worked pretty well for me!
- Badges
- Science topic
- Similar topics
- Biological Science
- Anatomy
- Tissue