Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Plant research: Watering uniformity/consistency. How important is it?

Trying to gather insight from an unresolved discussion with a colleague here, and either the available literature surrounding this topic and/or my keywords in search of are lacking. Context:...

21 February 2021 6,389 3 View

How to get rough estimation of the concentrations of Oxygen and moisture inside the glove box?

Dear colleagues, I was just wondering if you can suggest a good method to get a rough estimation to the concentration of O2 and H2O inside the glove box as unfortunately, our glove box doesn't...

17 February 2021 1,012 3 View

Onset of Photo Oxidation in Polymers?

Hi! I am looking for some specific information in regards to the minimum amount of UV intensity required to begin the photo degradation process in polymers. I understand each polymer is...

15 February 2021 7,703 4 View

Are you looking for a quality online conference on teaching and learning technology?

This large international conference will be held online this year due to COVID. I am submitting a paper to the Technology Enhanced Language Learning track (8). It is about a topic I have never...

15 February 2021 1,975 15 View

How do you calculate a discrimination index for polytomous data?

Hello, I'm currently running a PCA on a polytomous data set (5 point Likert). I want to create a discrimination index to narrow down my items before running the PCA. After looking around, I'm...

13 February 2021 3,517 2 View

Regarding immunology, transmissivity, herd immunity and vaccines in Sars CoV-2?

This is a follow-up on an earlier question regarding mechanistics of vaccines: why current vaccines don't block transmission. In summary, they don't engage mucosal immune cells to produce IgA....

08 February 2021 8,321 3 View

Which tropical country is the most easy for obtaining research permit (for biological research)?

Dear all, High beurocracy hihly complicate field ecolocial and evolutionary research nowadays. Nearly all tropical countries (maybe all?) requires research permit for performitng research and in...

04 February 2021 3,672 6 View

Can I polymerize NIPPAm with PEGmethacrylate or only with PEGacrylate?

I want to perform the synthesis of a hydrogel and I want to know if I can polymerize NIPPAm with PEGmethacrylate or only with PEGacrylate?

02 February 2021 2,106 6 View

What is the maximum storage time for samples used in soil enzyme assays?

01 February 2021 4,616 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How Can I bring as much common elements in steel as possible to calculate stable phase(s) in OpenCalphad?

As I was tweaking step-by-step OpenCalphad6 Macros step1.ocm involving phase and thermal property calculation of a High-speed steel, I could not incorporate more than 6 elements in the steel...

01 March 2021 8,840 1 View

How can we limit artifacts caused by rotation in fiber photometry?

We are trying to run fiber photometry experiments for long time scales, but we get these sinusoidal artifacts that come up. We think they may be caused by twisting of the fiber optic subject...

25 February 2021 568 1 View

What is the error of the Txy diagram?

Hi, I know the attached Txy diagram has an error but can't figure it out. Could you please look at the diagrams and explain it to me?

23 February 2021 4,239 1 View

How the values of errors can be calculated for thermodynamic data?

I have calculated thermodynamic parameters for surfactant solutions in aqueous and mixed alcoholic solvents. I want to calculate the value of errors in the data and represent it in the table along...

22 February 2021 239 2 View

Buckling analysis of a cylindrical shell in Ansys APDL?

In Ansys APDL, We are trying to validate our shell model for nonlinear buckling analysis considering the initial imperfection (as given by Ahmed et. al.) We have attached our results as attached....

13 February 2021 9,200 5 View

How to deal with cross loadings in EFA?

There is an issue of cross-loadings in my output results after running an EFA with varimax rotation. Should I ignore these cross-loadings since these differ by more than .20?

12 February 2021 802 4 View

Is it necessary to include orbital dynamics (translational) in attitude dynamics (rotational) problem?

I want to simulate satellite attitude control with a quaternion attitude representation. thrusters and reaction wheels are the main AOCS controllers. is it Ok to omit orbit dynamics (orbit...

31 January 2021 5,094 2 View

how to add blood and plasma in comsol?

I am trying to model separation of the plasma from the blood on a rotating microfluidic platform. I am not able to add blood and plasma in Comsol. someone please provide me your valuable...

27 January 2021 8,263 1 View

How to rotate an anisotropic stiffness matrix to an orthotropic matrix of cancellous bone?

Hi everyone, recently i have a question about how to calculate the orthotropic elastic matrix of cancellous bone cube. I really need the help!!!! I applied six stress states to the cancellous...

21 January 2021 9,143 2 View