how to know that our DNA sequence has the same species with one of DNA sequence from genebank in blast analysis? if the answer is the query cover and identity value must be 99% or more. then i have another question, i have a problem with my BLAST result. in top fifty species which have the closed homology with my DNA sequences are contain three different species name who has the same 99% identity value and query cover. so, which one i should choose as the same species with my DNA sequence? why it's happen? and this i show you my Blast result... >
you can check percent horology of You DNA with the BLAST result and check Minimum E- Value and high score
This happens many times in blast result for them who is working on fruit flies.
Above suggestions and comments by our friends are very good and can be try.
First , with new report and fruit fly taxonomist agreement, Bactrocera dorsalis and Bactrocera invadens are now one species i.e Bactrocera dorsalis. Whereas B. papaye is the member of Bactrocera dorsalis complex. therefor It may be giving the same blast match, E value and on other parameter.
I suggestion in this regard, you go back to the classical taxonomist and confirm your sample identity and submit your DNA sequences with Morphological identity of the species after careful decision on quality of the sequences.
Hope this may work
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