01 February 2017 3 3K Report

I am using a vibratome to cut coronal floating sections of brain tissue. I want to be able to cut the whole brain while keeping it together as a single section. I have been embedding in 3% agarose, but it does not hold the tissue together well. The parts of the brain that are not held together on their own float off as separate pieces. How can I section on the vibratome and ensure that my sections stay together as sections?

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