is it not enough with extracting total lipids (including membrane lipids) by using Folch or a similar method, and separate them subsequently by using TLC or SPE?? You will then have the isolated membrane lipids that can be further subjected to methylation for fatty acid analysis
https://www.researchgate.net/publication/220036753_Improvement_of_a_solid_phase_extraction_method_for_analysis_of_lipid_fractions_in_muscle_foods_Anal_Chim_Acta_5201-2201-205
Article Improvement of a solid phase extraction method for analysis ...
If you are extracting and methylating all the lipids, you might consider using direct methylation. We've used :
Rise ́ , P., Salvetti, F. and Galli, C. 2005. Application of a direct transmethylation method to the analysis of fatty acid profile in circulating and cultured cells. Anal. Biochem. 346: 182184.
An important trick for consistency is to add the red cells to methanol, mix, then add methanolic HCl. In addition, it's important to clean up the FAME by TLC before running on the GC.
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